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QI Xiaoyu, YE Yufei, CHEN Xuemei, ZHU Xiaonan, ZHANG Yuting, YIN Jian, WANG Xiaoli, ZHOU Nandi. Construction of targeted nanoprobe based on hyaluronic acid and its anti-tumor activity in vitro[J]. Journal of China Pharmaceutical University, 2022, 53(5): 542-553. DOI: 10.11665/j.issn.1000-5048.20220505
Citation: QI Xiaoyu, YE Yufei, CHEN Xuemei, ZHU Xiaonan, ZHANG Yuting, YIN Jian, WANG Xiaoli, ZHOU Nandi. Construction of targeted nanoprobe based on hyaluronic acid and its anti-tumor activity in vitro[J]. Journal of China Pharmaceutical University, 2022, 53(5): 542-553. DOI: 10.11665/j.issn.1000-5048.20220505
shu

Construction of targeted nanoprobe based on hyaluronic acid and its anti-tumor activity in vitro

  • 1.

    The Ministry of Education Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi 214122

  • 2.

    National Engineering Research Center for Function Food, Jiangnan University, Wuxi 214122, China

Funds: This study was supported by the National Natural Science Foundation of China (No.21877054)
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  • Received Date: April 26, 2022
  • Revised Date: June 26, 2022
    Graphical Abstract
    • Abstract
  • Hyaluronic acid (HA) was used as drug delivery carrier, and the doxorubicin (DOX), IR808 and catalase (CAT) were modified on hyaluronic acid (HA) to form the nano-probe CAT@HA-DOX-IR808 NPs with anti-tumor and fluorescence imaging by self-assembly.It was characterized by UV-Vis spectrophotometer, fluorescence spectrophotometer and transmission electron microscope, and its fluorescence imaging and antitumor activity were studied at solution and cell level.The experimental results showed that CAT@HA-DOX-IR808 NPs displayed a uniform near-spherical morphology with a size of 75 nm approximately.Under the condition of pH 5.0 + hyaluronidase (HAase), the release rate of DOX reached more than 80% in the first 10 hours. In the CD44 positive cells, laser confocal imaging results showed that the group of CAT@HA-DOX-IR808 NPs had more significant fluorescence signals than the group of free drugs and negative cell.In the cytotoxicity test, only about 40% of the MDA-MB-231 cells survived at the highest concentration of CAT@HA-DOX-IR808 NPs of the group of CAT@HA-DOX-IR808 NPs + NIR.Therefore, CAT@HA-DOX-IR808 NPs possess significantly enhanced anti-tumor effect with broad application prospect in the imaging and treatment of breast cancer in vitro.
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    • References (21)
  • [1]
    . FEBS J，2021，288（14）：4291-4310.
    [2]
    Aghamiri S，Zandsalimi F，Raee P，et al. Antimicrobial peptides as potential therapeutics for breast cancer［J］. Pharmacol Res，2021，171：105777.
    [3]
    Zhu YQ，Feijen J，Zhong ZY. Dual-targeted nanomedicines for enhanced tumor treatment［J］. Nano Today，2018，18：65-85.
    [4]
    Tavianatou AG，Piperigkou Z，Barbera C，et al. Molecular size-dependent specificity of hyaluronan on functional properties，morphology and matrix composition of mammary cancer cells［J］. Matrix Biol Plus，2019，3：100008.
    [5]
    Cai J，Fu JR，Li RR，et al. A potential carrier for anti-tumor targeted delivery-hyaluronic acid nanoparticles［J］. Carbohydr Polym，2019，208：356-364.
    [6]
    Matricardi P，di Meo C，Coviello T，et al. Interpenetrating Polymer Networks polysaccharide hydrogels for drug delivery and tissue engineering［J］. Adv Drug Deliv Rev，2013，65（9）：1172-1187.
    [7]
    Dzobo K，Sinkala M. Cancer stem cell marker CD44 plays multiple key roles in human cancers：immune suppression/evasion，drug resistance，epithelial-mesenchymal transition，and metastasis［J］. OMICS，2021，25（5）：313-332.
    [8]
    Zhang YB，Li Y，Tian HN，et al. Redox-responsive and dual-targeting hyaluronic acid-methotrexate prodrug self-assembling nanoparticles for enhancing intracellular drug self-delivery［J］. Mol Pharm，2019，16（7）：3133-3144.
    [9]
    Ashrafizadeh M，Mirzaei S，Gholami MH，et al. Hyaluronic acid-based nanoplatforms for Doxorubicin：a review of stimuli-responsive carriers，co-delivery and resistance suppression［J］. Carbohydr Polym，2021，272：118491.
    [10]
    Cui H，Huan ML，Ye WL，et al. Mitochondria and nucleus dual delivery system to overcome DOX resistance［J］. Mol Pharm，2017，14（3）：746-756.
    [11]
    Popescu RC，Savu DI，Bierbaum M，et al. Intracellular delivery of doxorubicin by iron oxide-based nano-constructs increases clonogenic inactivation of ionizing radiation in HeLa cells［J］. Int J Mol Sci，2021，22（13）：6778.
    [12]
    Hou WX，Zhao X，Qian XQ，et al. pH-Sensitive self-assembling nanoparticles for tumor near-infrared fluorescence imaging and chemo-photodynamic combination therapy［J］. Nanoscale，2016，8（1）：104-116.
    [13]
    Phua SZF，Yang GB，Lim WQ，et al. Catalase-integrated hyaluronic acid as nanocarriers for enhanced photodynamic therapy in solid tumor［J］. ACS Nano，2019，13（4）：4742-4751.
    [14]
    Feng L，Chen MY，Li RH，et al. Biodegradable oxygen-producing manganese-chelated metal organic frameworks for tumor-targeted synergistic chemo/photothermal/photodynamic therapy［J］. Acta Biomater，2022，138：463-477.
    [15]
    Li SP，Sun ZH，Deng GJ，et al. Dual-modal imaging-guided highly efficient photothermal therapy using heptamethine cyanine-conjugated hyaluronic acid micelles［J］. Biomater Sci，2017，5（6）：1122-1129.
    [16]
    Cai Y，Si WL，Huang W，et al. Organic dye based nanoparticles for cancer phototheranostics［J］. Small，2018，14（25）：e1704247.
    [17]
    Wang SJ，Tang QQ，Ya HY，et al. Study on the optical and biological properties in vitro of IR808‐PEG‐FA［J］. J Biomed Mater Res，2020，108（9）：1816-1823.
    [18]
    Leit?o MM，de Melo-Diogo D，Alves CG，et al. Prototypic heptamethine cyanine incorporating nanomaterials for cancer phototheragnostic［J］. Adv Healthc Mater，2020，9（6）：e1901665.
    [19]
    Liu SS，Song RX，Li XS，et al. Synergistic therapeutic strategies for cancer treatment based on nanophototherapy［J］. Nanophotonics，2021，10（12）：3391-3395.
    [20]
    Góth L. A simple method for determination of serum catalase activity and revision of reference range［J］. Clin Chim Acta，1991，196（2/3）：143-151.
    [21]
    Cheng X，He L，Xu JX，et al. Oxygen-producing catalase-based prodrug nanoparticles overcoming resistance in hypoxia-mediated chemo-photodynamic therapy［J］. Acta Biomater，2020，112：234-249.
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