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SUN Yimei, MAO Shihui, LI Lin, JIANG Weifeng, CHU Lisheng. Bone marrow mesenchymal stem cell-derived exosomes promote microglia/macrophage M2 polarization in acute cerebral ischemia rats and inhibit inflammatory response[J]. Journal of China Pharmaceutical University, 2023, 54(5): 599-606. DOI: 10.11665/j.issn.1000-5048.2023041703
Citation: SUN Yimei, MAO Shihui, LI Lin, JIANG Weifeng, CHU Lisheng. Bone marrow mesenchymal stem cell-derived exosomes promote microglia/macrophage M2 polarization in acute cerebral ischemia rats and inhibit inflammatory response[J]. Journal of China Pharmaceutical University, 2023, 54(5): 599-606. DOI: 10.11665/j.issn.1000-5048.2023041703
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Bone marrow mesenchymal stem cell-derived exosomes promote microglia/macrophage M2 polarization in acute cerebral ischemia rats and inhibit inflammatory response

  • School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China

Funds: This study was supported by the National Natural Science Foundation of China (No.81274113, No.81873028) and the Public Welfare Technology Application Research Project of Zhejiang Province (No.2016C33185)
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  • Received Date: April 16, 2023
  • Revised Date: October 17, 2023
    Graphical Abstract
    • Abstract
  • The aim of the present study was to investigate the effects of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on the polarization of M1/M2 microglia/macrophages in rats with acute cerebral ischemia.Ultrahigh-speed centrifugation was employed to isolate and identify exosomes; a middle cerebral artery occlusion (MCAO) model was prepared in rats using the intraluminal filament technique; Longa scoring and corner tests were used to evaluate the neurological function of rats; 2, 3, 5-triphenyltetrazole chloride (TTC) staining was used to assess the infarct volume in rat brains; immunofluorescence double-labeling of CD16/32/Iba1 and CD206/Iba1 was performed to detect M1/M2 phenotypes of microglia/macrophages; RT-qPCR was employed to measure the mRNA expression of CD86, inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNF-α), arginase-1 (Arg-1), interleukin-10 (IL-10), and transforming growth factor beta (TGF-β) in the ischemic penumbra of rat brains.The experimental results showed that BMSC-Exos reduced the number of CD16/32+/Iba1+ positive cells in the ischemic penumbra (P < 0.01) while increasing the number of CD206+/Iba1+positive cells (P < 0.01), and decreased the mRNA expression of iNOS, CD86, and TNF-α, while increasing the mRNA expression of Arg-1, TGF-β, and IL-10 (P < 0.05 or P < 0.01).This research suggests that BMSC-Exos can regulate M1/M2 polarization of microglia/macrophages in rats with acute cerebral ischemia, alleviate neuroinflammation, and improve ischemic brain injury.
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    • References (27)
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