Effects of rare ginsenoside on idiopathic pulmonary fibrosis

YAO Lei; QU Linlin; FAN Daidi

doi:10.11665/j.issn.1000-5048.2023042002

Journal of China Pharmaceutical University > 2023 > 54(5): 607-613. > DOI: 10.11665/j.issn.1000-5048.2023042002

YAO Lei, QU Linlin, FAN Daidi. Effects of rare ginsenoside on idiopathic pulmonary fibrosis[J]. Journal of China Pharmaceutical University, 2023, 54(5): 607-613. DOI: 10.11665/j.issn.1000-5048.2023042002

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Effects of rare ginsenoside on idiopathic pulmonary fibrosis

YAO Lei^1,2,
QU Linlin^1,2,
FAN Daidi^1,2

1.
School of Chemical Engineering,Northwest University, Xi'an 710069
2.
Biotech &Biomed Research Institute,Northwest University, Xi'an 710069, China

Funds: This study was supported by the National Key Research and Development Program of China (No.2021YFC2101500), and Key Research and Development Program of Shaanxi Province (No.2022ZDLSF05-12)

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Received Date: April 19, 2023
Revised Date: September 17, 2023

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Abstract

Abstract

To investigate whether rare ginsenosides could alleviate idiopathic pulmonary fibrosis (IPF), C57BL/6 mice were randomly divided into control group, bleomycin (BLM)-induced IPF group, rare ginsenoside Rk1 group, rare ginsenoside Rk3 group, rare ginsenoside Rh4 group and rare ginsenoside Rg5 group.All mice except those in the control group were given bleomycin injection.The IPF model was established by BLM for 28 days.The treatment group was given ginsenoside intragastrically at the same time.After the experiment, the lung tissues of mice were collected and the pathological changes of the mice lungs were observed.The content of hydroxyproline (HYP) in mouse lung tissue was measured.The expression of IPF-related genes in mouse lung tissues was detected.In in vitro experiments, Medical Research Council cell strain-5 (MRC-5) was used to induce IPF cell model using transforming growth factor-β1 (10 ng/mL).The effects of four saponins on the expression of IPF-related genes were analyzed by MTT assay, HYP content determination and RT-qPCR.All four rare ginsenosides could effectively alleviate the pathological process such as alveolar structure destruction caused by IPF, reduce the content of HYP, and down-regulate the expression of IPF-related genes, indicating that rare ginsenosides can effectively alleviate IPF.
- pulmonary fibrosis,
- rare ginsenoside,
- MRC-5 cell,
- transforming growth factor β1,
- bleomycin

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References

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