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WANG Deng, SUN Zhongying, GUO Qinglong, et al. Therapeutic effect and mechanism of the topical preparation of baicalein on atopic dermatitis[J]. J China Pharm Univ, 2025, 56(1): 99 − 109. DOI: 10.11665/j.issn.1000-5048.2024070302
Citation: WANG Deng, SUN Zhongying, GUO Qinglong, et al. Therapeutic effect and mechanism of the topical preparation of baicalein on atopic dermatitis[J]. J China Pharm Univ, 2025, 56(1): 99 − 109. DOI: 10.11665/j.issn.1000-5048.2024070302

Therapeutic effect and mechanism of the topical preparation of baicalein on atopic dermatitis

Funds: This study was supported by the National Natural Science Foundation of China (No. 82002907); Zaozhuang Talent Gathering Project; Taishan Industry Leading Talent Project; and the Guiding Funds of Central Government for Supporting the Development of the Local Science and Technology of Shandong Province (YDZX2023082)
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  • Received Date: July 02, 2024
  • Revised Date: August 29, 2024
  • Accepted Date: September 04, 2024
  • To evaluate the therapeutic effect of baicalein topical preparation on atopic dermatitis, we first constructed two atopic dermatitis-like mouse models induced by calcipotriol and 1-fluoro-2,4-dinitrobenzene to assess their therapeutic effect with skin tissue staining and other experiments. It was found that topical preparation of baicalein could alleviate epidermal thickening of diseased skin tissues, repair damaged skin barrier proteins, and inhibit T helper 2 cells (Th2) infiltration and mast cell infiltration and activation in lesional sites. Cyberpharmacology was utilized to analyze whether baicalein could treat atopic dermatitis by interfering with multiple pathogenesis-associated pathways. Results indicated that baicalein reduced the mRNA levels of inflammatory factors and inhibited the phosphorylation of NF-κB p65 and STAT1 proteins in keratinocyte cells. Together, the topical preparation of baicalein may be effective in alleviating atopic dermatitis-like symptoms in mice by down-regulating the phosphorylation level of NF-κB in keratinocytes, thereby decreasing the expression of inflammatory factors in keratinocytes, which provides an idea and a theoretical basis for the topical preparation of baicalein for the treatment of inflammatory skin diseases such as atopic dermatitis.

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