Effect of the Jiangya Yishen granule on regulative system of extracellular matrix decomposition of spontaneously hypertensive rats

This article investigate the effect of the Jiangya Yishen（JYYS） granule on regulative system of extracellular matrix（ECM）decomposition of spontaneously hypertensive rats（SHR）,we determined the expression of include transforming growth factor β₁（TGF-β₁）,collagen type IV（COLIV）,matrix metalloproteinase-9（MMP-9）,tissue inhibitor of metalloproteinase-1（TIMP-1）by utilizing real time fluorescent PCR technique (RT-PCR) and laser confocal fluorescence microscopy.The results showed that JYYS could enhance the level of renal MMP-9 mRNA and its protein level,meanwhile down regulate the transcription of TIMP-1,TGF-β₁,COLIV and decreasing their protein level after long term treatment.Therefore,JYYS corrected the imbalance of MMP-9/TIMP-1,then enhanced the degradation of ECM and decreased the accumulation of ECM to protect kidney in SHR.