Hypoglycemic effect of<i> Lactococcus lactis</i> vaccine containing HSP65-6P277 on streptozotocin-induced type 1 diabetic mice

WU Jie; LIU Xiaorui; YANG Xue; HOU Jing; XU Maolei; SHEN Lili; HUANG Dongcheng; LIU Kunfeng

doi:10.11665/j.issn.1000-5048.20140120

Journal of China Pharmaceutical University > 2014 > 45(1): 106-110. > DOI: 10.11665/j.issn.1000-5048.20140120

WU Jie, LIU Xiaorui, YANG Xue, HOU Jing, XU Maolei, SHEN Lili, HUANG Dongcheng, LIU Kunfeng. Hypoglycemic effect of Lactococcus lactis vaccine containing HSP65-6P277 on streptozotocin-induced type 1 diabetic mice[J]. Journal of China Pharmaceutical University, 2014, 45(1): 106-110. DOI: 10.11665/j.issn.1000-5048.20140120

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Hypoglycemic effect of Lactococcus lactis vaccine containing HSP65-6P277 on streptozotocin-induced type 1 diabetic mice

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Abstract

To study the hypoglycemic effect and mechanisms of vaccine HSP65-6P277 protein on type 1 diabetic mice induced by streptozotocin(STZ), Lactococcus lactis live vector was used to express the vaccine protein. The mouse model of type 1 diabetes mellitus were established by intraperitoneal injections of multiple low dose STZ(MLD-STZ)and divided into three groups. The inducible expression strain L. lactis NZ9000 pCYT ∶HSP65-6P277, constitutive expression strain L. lactis NZ9000 pHJ ∶HSP65-6P277 and control strains L. lactis NZ9000 pCYT ∶Nuc were respectively immunized to diabetic mice orally once daily(2×10⁹ CFU, 200 μL)in the first week and once every week in the following weeks lasting for 16 weeks. Fasting blood glucose levels were measured once a month on blood samples taken from orbital venous plexus. Four months later, the mice were sacrificed. Spleen cell proliferation experiment was performed. IL-10 and IFN-γ cytokine levels of spleen cells and serum IgG levels were assayed using ELISA kits. The results showed that pCYT ∶HSP65-6P277 group and pHJ ∶HSP65-6P277 group have low blood glucose(P < 0. 05), and maintain normal body weight, compared with control group(pCYT ∶Nuc). On the other hand, cytokine IFN-γ levels were significantly lower(P < 0. 05)in spleen cells supernatant and spleen cell proliferation was reduced against HSP65 and P277(P< 0. 05), but serum IgG levels were not significantly different(P> 0. 05).
- Lactococcus lactis,
- vaccine,
- P277,
- mucosal immune,
- type 1 diabetic mellitus

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Hypoglycemic effect of Lactococcus lactis vaccine containing HSP65-6P277 on streptozotocin-induced type 1 diabetic mice

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