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KANG An, ZHANG Shengjie, SHAN Jinjun, DI Liuqing. Pharmacokinetics of ginsenoside Rb1 in lincomycin-induced gut microbiota dysbiosis rats[J]. Journal of China Pharmaceutical University, 2016, 47(2): 182-187. DOI: 10.11665/j.issn.1000-5048.20160210
Citation: KANG An, ZHANG Shengjie, SHAN Jinjun, DI Liuqing. Pharmacokinetics of ginsenoside Rb1 in lincomycin-induced gut microbiota dysbiosis rats[J]. Journal of China Pharmaceutical University, 2016, 47(2): 182-187. DOI: 10.11665/j.issn.1000-5048.20160210
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Pharmacokinetics of ginsenoside Rb1 in lincomycin-induced gut microbiota dysbiosis rats

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  • Gut microbiota-mediated deglycosylationplays an important role in the metabolism of ginsenoside Rb1. Thus, a lincomycin-induced gut microbiota dysbiosis rat model was selected to explored the pharmacokinetics and deglycosylation metabolism of ginsenoside Rb1. An UPLC-MS/MS analytical method was developed to detect ginsenoside Rb1 and its deglycosylated metabolite, Rd in rat plasma. The triple quadruple mass spectrometer was set in negative electrospray ionization mode by multiple reaction monitoring. The method was validated to meet the requirements of biological applications, by evaluating specificity, linearity, lower limits of quantification(LLOQ), precision, accuracy, matrix effect, recovery and stability. Gut microbiota dysbiosis rats were induced by oral administration of lincomycin(5 000 mg/kg)for 7 continuous days. The in vitro and in vivo results reveal that the reduced β-D-glucosidase activity significantly decreases the Rd formation rate in lincomycin-induced gut microbiota dysbiosis rats, leading to the pharmacokinetic alteration of ginsenoside Rb1 and Rd in gut microbiota dysbiosis rats.
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    • References (10)
  • [1]
    Kang A, Hao H, Zheng X, et al. Peripheral anti-inflammatory effects explain the ginsenosides paradox between poor brain distribution and anti-depression efficacy[J].J Neuroinflammation,2011,8:100.
    [2]
    Hao K,Gong P,Sun SQ,et al.Beneficial estrogen-like effects of ginsenoside Rb1,an active component of Panax ginseng,on neural 5-HT disposition and behavioral tasks in ovariectomized mice[J].Eur J Pharmacol,2011,659(1):15-25.
    [3]
    Liu C,Zhang M,Hu MY,et al.Increased glucagon-like peptide-1 secretion may be involved in antidiabetic effects of ginsenosides[J].J Endocrinol,2013,217(2):185-196.
    [4]
    Hu Z,Yang J,Cheng C,et al.Combinatorial metabolism notably affects human systemic exposure to ginsenosides from orally administered extract of Panax notoginseng roots(Sanqi)[J].Drug Metab Dispos,2013,41(7):1457-1469.
    [5]
    Zhou L,Xing R,Xie L,et al.Development and validation of an UFLC-MS/MS assay for the absolute quantitation of nine notoginsenosides in rat plasma:application to the pharmacokinetic study of Panax notoginseng extract[J].J Chromatogr B Analyt Technol Biomed Life Sci,2015,995-996:46-53.
    [6]
    Li X,Wang G,Sun J,et al.Pharmacokinetic and absolute bioavailability study of total panax notoginsenoside,a typical multiple constituent traditional Chinese medicine(TCM)in rats[J].Biol Pharm Bull,2007,30(5):84-88.
    [7]
    Yan Q,Zhou W,Shi XL,et al.Biotransformation pathways of ginsenoside Rb1 to compound K by β-glucosidases in fungus Paecilomyces bainier sp.229[J].Pro Biochem,2010,45(9):1550-1556.
    [8]
    Sullivan A, Barkholt L, Nord CE. Lactobacillus acidophilus,Bifidobacterium lactis and Lactobacillus F19 prevent antibiotic-associated ecological disturbances of Bacteroides fragilis in the intestine[J].J Antimicrob Chemother,2003,52(2):308-311.
    [9]
    Jang SE,Jung IH,Joh EH,et al.Antibiotics attenuate anti-scratching behavioral effect of ginsenoside Re in mice[J].J Ethnopharmacol,2012,142(1):105-112.
    [10]
    Zhang SJ, Guo JR, Kang A, et al. Advance in studies on gut microbiota in de-glycosylation of traditional Chinese medicine glycosides[J].Chin J Chin Mater Medica(中国中药杂志),2013,38(10):1459-1466.
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