• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
HUI Renjie, HE Xu, LIU Jingyi, FENG Jing, ZENG Shuai, FENG Bainian. Rapid determination of six flavonoids from seabuckthorn leaves by RP-HPLC-DAD[J]. Journal of China Pharmaceutical University, 2017, 48(6): 696-700. DOI: 10.11665/j.issn.1000-5048.20170610
Citation: HUI Renjie, HE Xu, LIU Jingyi, FENG Jing, ZENG Shuai, FENG Bainian. Rapid determination of six flavonoids from seabuckthorn leaves by RP-HPLC-DAD[J]. Journal of China Pharmaceutical University, 2017, 48(6): 696-700. DOI: 10.11665/j.issn.1000-5048.20170610
shu

Rapid determination of six flavonoids from seabuckthorn leaves by RP-HPLC-DAD

More Information
    Graphical Abstract
    • Abstract
  • To establish a rapid determination method of six flavonoids: catechin, rutin, myricetin, quercetin, kaempferol and isorhamnetin, from seabuckthorn leaves by RP-HPLC-DAD. The seabuckthorn leaves were first degreased by petroleum ether, extracted by ethanol, and determined by RP-HPLC-DAD. The six flavonoids were separated and eluted by a Shimadzu C18(150 mm×2. 1 mm, 5 μm)column with methanol-water(0. 1% phosphoric acid)(60∶40)at a flow rate of 1. 0 mL/min. The detection wavelength were as follow: catechin 208 nm, rutin 257 nm, myricetin 373 nm, quercetin 371 nm, kaempferol 367 nm, and isorhamnetin 371 nm, respectively. The injection volume was 20 μL. The contents of the six flavonoids were in the range of 0. 47 to 30. 00 μg/mL with good linearity. The validation of the method, including precision, stability and recovery rate, was acceptable. The established method can be used for fast determination of the content of six flavonoids in seabuckthorn leaves.
    • FullText(HTML)
    • References (15)
  • [1]
    Liu R,Zhang C.Advances in the research of the chemical components of seabuckthorn[J].J Shanxi Datong Univ(Nat Sci Edition)[山西大同大学学报(自然科学版)],2009,25(2):43-44,54.
    [2]
    Guliyev VB,Gul M,Yildirim A.Hippophae rhamnoides L.:chromatographic methods to determine chemical composition,use in traditional medicine and pharmacological effects[J].J Chromatogr B,2004,812(1/2):291-307.
    [3]
    Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1(中华人民共和国药典)[S].Beijing:China Medical Science Press,2015:184-185.
    [4]
    Liu Y,Lian YS,Wang YL,et al.Review of research and development and significant effect of Hippophae rhamnoides[J].Chin J Chin Materia Medica(中国中药杂志),2014,39(9):1547-1552.
    [5]
    Yogendra Kumar MS,Tirpude RJ,Maheshwari DT,et al.Antioxidant and antimicrobial properties of phenolic rich fraction of seabuckthorn(Hippophae rhamnoides L.)leaves in vitro[J].Food Chem,2013,141(4):3443-3450.
    [6]
    Pallavi B, Chandresh V, Kanika K, et al. In vitro evaluation of antidiabetic and antioxidant activity of seabuckthorn(Hippophae rhamnoides L.)leaves[J].J Med Plant Res,2015,9(35):929-932.
    [7]
    Bao M,Lou Y.Flavonoids from seabuckthorn protect endothelial cells(EA.hy926)from oxidized low-density lipoprotein induced injuries via regulation of LOX-1 and eNOS expression[J].J Cardiovasc Pharmacol,2006,48(1):834-841.
    [8]
    Cheng J,Kondo K,Suzuki Y,et al.Inhibitory effects of total flavones of Hippophae rhamnoides L.on thrombosis in mouse femoral artery and in vitro platelet aggregation.[J].Life Sci,2003,72(20):2263-2271.
    [9]
    Ganju L,Padwad Y,Singh R,et al.Anti- inflammatory activity of seabuckthorn(Hippophae rhamnoides)leaves[J].Int J Immunopharmacol,2005,5(12):1675-1684.
    [10]
    Geetha S,Ram MS,Sharma SK,et al.Cytoprotective and antioxidant activity of seabuckthorn(Hippophae rhamnoides L.)flavones against tert-butyl hydroperoxide-induced cytotoxicity in lymphocytes.[J].J Med Food,2009,12(1):151-158.
    [11]
    Zhou JY,Zhou SW,Du XH,et al.Protective effect of total flavonoids of seabuckthorn(Hippophaerhamnoides)in simulated high-altitude polycythemia in rats[J].Molecules,2012,17(10):11585-11597.
    [12]
    Rösch D,Krumbein A,Mügge,et al.Structural investigations of flavonol glycosides from sea buckthorn(Hippophaë rhamnoides)pomace by NMR spectroscopy and HPLC-ESI-MSn[J].J Agric Food Chem,2004,52(13):4039-4046.
    [13]
    Wei G,Hou X.HPLC method in determining the content of quercetin,kaempferide and isorhamnetin in seabuckthorn flavone powder[J].Global Seabuckthorn Res Dev(国际沙棘研究与开发),2014,12(2):22-24.
    [14]
    Hui R,Feng J,Ling M,et al.Multicriteria comprehensive assessment on the optimization of extraction technology of Hippophae rhamnoides Linn.flavonoids from leaves and seeds[J].China Pharm(中国药房),accepted.
    [15]
    Du L,Zhang H,Xiao W,et al.Comparative determination of total flavonoids in seabuckthron leaves from 10 species by RP-HPLC[C].The Fifth Shanghai International Conference on Traditional Chinese Medicine and Natural Medicine.2012:281-285.
    • Related Articles

  • Related Articles

    [1]SONG Linqi, ZHENG Feng. Preparation, characterization and in vitro release of diclofenac grafted with hyaluronic acid[J]. Journal of China Pharmaceutical University, 2025, 56(1): 49-55. DOI: 10.11665/j.issn.1000-5048.2024072601
    [2]WANG Wendie, ZHOU Jiyuan, TANG Lidan, YIN Tingjie. Preparation and characterization of dasatinib albumin micelles[J]. Journal of China Pharmaceutical University, 2022, 53(3): 286-292. DOI: 10.11665/j.issn.1000-5048.20220305
    [3]WANG Xiaoying, WANG Xiaying, QIU Liangzhen, OUYANG Huizhi, XU Wei. Cytotoxicity and cellular uptake of paclitaxel-loaded carboxymethyl chitosan-rhein polymeric micelles in MCF-7 cells[J]. Journal of China Pharmaceutical University, 2020, 51(1): 33-37. DOI: 10.11665/j.issn.1000-5048.20200106
    [4]LIU Zhangmin, CHAO Jiajia, FENG Yan, LI Qian, CUI Li. Characterization of glutamate dehydrogenase SHJG_7666 from Streptomyces hygroscopicus 5008[J]. Journal of China Pharmaceutical University, 2018, 49(3): 360-368. DOI: 10.11665/j.issn.1000-5048.20180316
    [5]SUN Jingfang, GUO Chunjing, ZHANG Yunduan, SONG Xiaoyan, LYU Jiantao, YIN Jungang, CHEN Daquan. Preparation and characterization of multi-functional targeting nano-carrier based on pH-redox tumor microenvironment[J]. Journal of China Pharmaceutical University, 2017, 48(3): 305-310. DOI: 10.11665/j.issn.1000-5048.20170309
    [6]LIAN Shengnan, SUN Jingfang, SUN Kaoxiang, LIU Wanhui, CHEN Daquan. Preparation and characterization of oligomeric hyaluronic acid-ketal as a tumour CD44 receptor and pH-sensitive targeting carrier[J]. Journal of China Pharmaceutical University, 2014, 45(5): 540-543. DOI: 10.11665/j.issn.1000-5048.20140506
    [7]SONG Yiting, LIAO Jiao, QI Xuan, GAO Yuan, ZHANG Jianjun. Physicochemical characterization of co-amorphous repaglinide-saccharin[J]. Journal of China Pharmaceutical University, 2013, 44(5): 427-431. DOI: 10.11665/j.issn.1000-5048.20130508
    [8]WANG Jing, GAN Li, GAN Yong, LIU Jianping. Preparation and characterization of hyaluronic acid-modified dexamethasone core-shell liponanoparticles[J]. Journal of China Pharmaceutical University, 2013, 44(2): 117-123. DOI: 10.11665/j.issn.1000-5048.20130204
    [9]Characterization of N-terminal PEGylated Endostar[J]. Journal of China Pharmaceutical University, 2010, 41(5): 476-480.
    [10]Preparation and Characterization of Novel Galactosylated Chitosan[J]. Journal of China Pharmaceutical University, 2003, (5): 1-4.

Catalog

    Get Citation
    PDF
    XML
    Article views (886) PDF downloads (1615) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：297132

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return