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AN Jiong, LI Kan, XU Xiaoming, GAO Zhun, HU Chao, LI Bo, DI Bin, XU Lili. Development and validation of a novel and simple RP-HPLC method for the determination of related substances of tiopronin for injection[J]. Journal of China Pharmaceutical University, 2019, 50(3): 317-324. DOI: 10.11665/j.issn.1000-5048.20190307
Citation: AN Jiong, LI Kan, XU Xiaoming, GAO Zhun, HU Chao, LI Bo, DI Bin, XU Lili. Development and validation of a novel and simple RP-HPLC method for the determination of related substances of tiopronin for injection[J]. Journal of China Pharmaceutical University, 2019, 50(3): 317-324. DOI: 10.11665/j.issn.1000-5048.20190307
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Development and validation of a novel and simple RP-HPLC method for the determination of related substances of tiopronin for injection

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  • In this paper, a novel and simple RP-HPLC method for the determination of related substances of tiopronin for injection was described. The RP-HPLC analysis was performed on a C18 column, with acetonitrile-0. 1% phosphoric acid(8 ∶92), mobile phase in isocratic mode at a rate of 1. 0 mL/min. The photodiode array detector was set at 210 nm. Seven related substances were detected and the structures were characterized by mass spectrometry. The method showed great suitability, specificity and excellent linearity over the concentration range of 0. 3 to 50 μg/mL(r≥0. 999), and the limits of detection and quantitation were found to be 0. 10 and 0. 31 μg/mL, respectively. The accuracy of the method determined by the entire mean recovery ranged from 98. 7% to 103. 7%. The intra-and inter-day precision was satisfactory(RSD≤4. 4%)and robust(RSD≤6. 4%). And this method was successfully applied for the determination of related substances of tiopronin for injection, which revealed the retention of sulfhydryl compounds and glycine analogues on the RP-HPLC and the effect of the pH value of the mobile phase on the chromatographic behavior of the analytes.
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  • [1]
    Wang JQ,Zou YH,Huang C,et al.Protective effects of tiopronin against high fat diet-induced non-alcoholic steatohepatitis in rats[J].Acta Pharmacol Sin,2012,33(6):791-797.
    [2]
    Tasic V,Lozanovski VJ,Ristoska-Bojkovska N,et al.Nephrotic syndrome occurring during tiopronin treatment for cystinuria[J].Eur J Pediatr,2011,170(2):247-249.
    [3]
    Amor B,Mery C,De GA.Tiopronin(N-[2-mercaptopropionyl] glycin)in rheumatoid arthritis[J].Arthritis Rheum,2014,25(6):698-703.
    [4]
    Ichikawa H,Imaizumi K,Tazawa Y,et al.Effect of tiopronin on senile cataracts[J].Ophthalmologica,1980,180(5):293-298.
    [5]
    Zhang JG,Viale M,Esposito M,et al.Tiopronin protects against the nephrotoxicity of cisplatin in the rat[J].Hum Exp Toxicol,1999,18(12):713-717.
    [6]
    Tomás Pérez-Ruiz, Martínez-Lozano C, Baeyens WRG, et al.Determination of tiopronin in pharmaceuticals using a chemiluminescent flow-injection method[J].J Pharm Biomed Anal,1998,17(4/5):823-828.
    [7]
    Devi PU,Saini MR,Saharan BR,et al.Radioprotective effect of 2-mercaptopropionylglycine on the intestinal crypt of Swiss albino mice after Cobalt-60 irradiation[J].Radiat Res,1979,80(1):214-220.
    [8]
    Kurumaji Y,Miyazaki K.Tiopronin-induced lichenoid eruption in a patient with liver disease and positive patch test reaction to drugs with sulfhydryl group[J].J Dermatol,1990,17(3):176-181.
    [9]
    Kurosaki M,Takagi H,Mori M.HLA-A33/B44/DR6 is highly related to intrahepatic cholestasis inducedby tiopronin[J].Digest Dis Sci,2000,45(6):1103-1108.
    [10]
    Shichiri M.Primary biliary cirrhosis:a patient with adverse reactions to tiopronin and autoimmune hemolyticanemia with reticulocytopenia[J].Arch Intern Med, 1984,144(1):89-91.
    [11]
    Ding XL,Lei ZB.Literature review of 38 cases with tiopronininduced anaphylatic shock[J].Adverse Drug React J(药物不良反应杂志),2005(5):336-339.
    [12]
    Leroy P,Nicolas A,Gavriloff C,et al.Determination of 2-mercaptopropionylglycine and its metabolite,2-mercaptopropionic acid,in plasma by ion-pair reversed-phase high-performance liquid chromatography with post-column derivatization[J].J Chromatogr B,1991,564(1):258-265.
    [13]
    Wang B, Zhao L, Wang H, et al. Determination of tiopronin in human plasma and pharmacokinetics by LC-ESI-MS[J].Chromatographia,2009,70(1/2):89-94.
    [14]
    Matsuura K,Takashina H.Gas chromatographic-mass spectrometric determination of tiopronin in human blood using acrylic acid esters as a derivatization reagent for the thiol group[J].J Chromatogr B,1993,616(2):229-234.
    [15]
    Xu J,Cai R,Wang J,et al.Fluorometric assay of tiopronin based on inhibition of multienzyme redox system[J].J Pharm Biomed Anal,2005,39(1/2):334-338.
    [16]
    Murillo Pulgarín,José A,Lemus Gallego,et al.Determination of tiopronin in pharmatceutical preparations by time resolved chemiluminescence using the stopped-flow technique[J].Anal Lett,2013,46(11):1836-1848.
    [17]
    Li Q,Gao L.Spectrophotometric determination of tiopronin using its catalytic reaction between sodium 1,2-naphthoquinone-4-sulfonate and hydroxyl ion[J].Anal Sci,2009,25(1):89-93.
    [18]
    Casassas E,Ariño C,Esteban M,et al.Analytical determination of N-(2-mercapto propionyl)glycine(tiopronin)by voltammetric methods[J].Anal Lett,2006,24(7):1183-1199.
    [19]
    Nelson D, Cox M. Lehninger principles of biochemistry(4th Ed.)[J].Biochem Mol Biol Edu,2005,33(1):74-75.
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