Effects of different immunogenic amino acids in PD-L1 vaccine on the differentiation of T cell subsets

CHEN Hongmei; KANG Yanliang; LIU Li; YAO Wenbing; TIAN Hong

doi:10.11665/j.issn.1000-5048.20200313

Journal of China Pharmaceutical University > 2020 > 51(3): 349-356. > DOI: 10.11665/j.issn.1000-5048.20200313

CHEN Hongmei, KANG Yanliang, LIU Li, YAO Wenbing, TIAN Hong. Effects of different immunogenic amino acids in PD-L1 vaccine on the differentiation of T cell subsets[J]. Journal of China Pharmaceutical University, 2020, 51(3): 349-356. DOI: 10.11665/j.issn.1000-5048.20200313

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Effects of different immunogenic amino acids in PD-L1 vaccine on the differentiation of T cell subsets

Funds: This study was supported by the National Natural Science Foundation of China (No. 81973222, No.81673343, No.91753112)

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Received Date: March 22, 2020
Revised Date: May 07, 2020

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Abstract

Abstract

To compare the effects of endogenous 3-nitrotyrosine and non-natural 4-nitrophenylalanine in PD-L1 vaccine on the differentiation of T cell subsets, two immunogenic amino acids were introduced into the same site of PD-L1 vaccine. Two PD-L1 mutants with 3-nitrotyrosine and 4-nitrophenylalanine were obtained, respectively, using genetic code expansion technology. Mice were immunized with these two mutants, and their effects on the differentiation of T cell subsets in spleen were analyzed. The results of flow cytometry showed that the introduction of 4-nitrophenylalanine in PD-L1 vaccine could promote the polarization of Th1 cells while reducing the proportion of Treg cells; the introduction of 3-nitrotyrosine had no effect on the polarization of Th1 cells, while significantly increasing the proportion of Treg and Th17 cells. The introduction of both into PD-L1 vaccine could promote the response of CD8⁺ T cells in spleen, and the response of PD-L1 mutant containing 4-nitrophenylalanine was stronger. In summary, the non-natural 4-nitrophenylalanine is more suitable for the design of tumor vaccines as compared with endogenous 3-nitrotyrosine.
- PD-L1,
- vaccine,
- 3-nitrotyrosine,
- 4-nitrophenylalanine,
- T cell differentiation

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