Research progress of next-generation gene editing tools

WANG Rui; ZHOU Xinjie; DU Xiqin; HAO Di; WANG Chen; ZOU Bingjie; SONG Qinxin; ZHOU Guohua

doi:10.11665/j.issn.1000-5048.20220601

Journal of China Pharmaceutical University > 2022 > 53(6): 633-642. > DOI: 10.11665/j.issn.1000-5048.20220601

WANG Rui, ZHOU Xinjie, DU Xiqin, HAO Di, WANG Chen, ZOU Bingjie, SONG Qinxin, ZHOU Guohua. Research progress of next-generation gene editing tools[J]. Journal of China Pharmaceutical University, 2022, 53(6): 633-642. DOI: 10.11665/j.issn.1000-5048.20220601

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Research progress of next-generation gene editing tools

1.
Key Laboratory of Drug Quality Control and Pharmacovigilance of the Ministry of Education, China Pharmaceutical University, Nanjing 210009
2.
Department of Chemistry,State Key Laboratory of Analytical Chemistry for Life Science,Nanjing University, Nanjing 210093
3.
Department of Clinical Pharmacy, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China

Funds: This study was funded by the National Natural Science Foundation of China (No.82173780), the Open Project Program of MOE Key Laboratory of Drug Quality Control and Pharmacovigilance (No.DQCP20/21MS04); National Innovation and Entrepreneurship Training Program for Undergraduate (No.202210316001Z and 202210316009Z)

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Received Date: December 03, 2022
Revised Date: December 19, 2022

Graphical Abstract

Abstract

Abstract

Gene editing tools with nucleases as the main component have now implemented programmable targeted mutagenesis or insertion or deletion of mammalian genomes.From zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), CRISPR/Cas system to safer and more accurate Cas9 fusion protein gene editing tools and other nuclease gene editing tools, this paper systematically describes the development and evolution of gene editing, with detailed introduction to the development and optimization of next-generation gene editing tools, and a prospect of the clinical application of and challenges for gene editing tools.
- gene editing,
- CRISPR/Cas9,
- base editing,
- prime editing,
- double-strand break,
- progress

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References (43)

References

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