Advances in the preparation and structural characterization of rhamnogalacturonan II

LIU Yupeng; SHI Songshan; BU Yingxuan; GONG Huan; WANG Huijun; WANG Shunchun

doi:10.11665/j.issn.1000-5048.2024040202

Journal of China Pharmaceutical University > 2024 > 55(4): 432-442. > DOI: 10.11665/j.issn.1000-5048.2024040202

LIU Yupeng, SHI Songshan, BU Yingxuan, et al. Advances in the preparation and structural characterization of rhamnogalacturonan II[J]. J China Pharm Univ, 2024, 55(4): 432 − 442. DOI: 10.11665/j.issn.1000-5048.2024040202

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Advances in the preparation and structural characterization of rhamnogalacturonan II

LIU Yupeng^{1, #},
SHI Songshan^{1, #},
BU Yingxuan¹,
GONG Huan¹,
WANG Huijun^{1, 2, ,},
WANG Shunchun^{1, 2, ,}

1.
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203
2.
Key Laboratory of Chinese Medicine Standardization, Ministry of Education, Shanghai 201203, China

Funds: This study was supported by the Natural Science Foundation of Shanghai(No.21ZR1462900),the National Natural Science Foundation of China(No.82274078，No.U23A20512), and Shanghai Oriental Talent Program for Youth

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Received Date: April 01, 2024

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Abstract

Abstract

Rhamnogalacturonan II (RG-II) is one of the structural domains of pectin whose structure is highly conserved among species. The main chain of RG-II consists of approximately nine galacturonic acids linked by α-1,4 glycosidic bonds, with six well-defined side chains replacing them (A−F). The structures of the disaccharide side chains C and D and the monosaccharide side chains E and F in RG-II from different sources remain essentially the same. In contrast, the oligosaccharide side chains A and B showed slight variability. Structural characterization of RG-II can be achieved by molecular weight, monosaccharide composition, and mass spectrometry. The polysaccharides containing RG-II structural domains in traditional Chinese medicines (TCMs) have high medicinal value. Isolation of RG-II can be achieved using endo-polygalacturonase (Endo-PG) and Penicillium oxalicum. A substantial number of RG-II standards can be rapidly prepared from red wine for the development of new quantitative methods to realize the quality control of active polysaccharides from traditional Chinese medicines and to promote the research process of polysaccharides from traditional Chinese medicines.
- pectin,
- rhamnogalacturonan II,
- RG-II,
- endo-polygalacturonase,
- penicillium oxalate,
- red wine

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References

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