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Cloning of Spinach Glycolate Oxidase cDNA and Its Construction into Yeast Expression Vector[J]. Journal of China Pharmaceutical University, 2003, (1): 83-86.
Citation: Cloning of Spinach Glycolate Oxidase cDNA and Its Construction into Yeast Expression Vector[J]. Journal of China Pharmaceutical University, 2003, (1): 83-86.
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Cloning of Spinach Glycolate Oxidase cDNA and Its Construction into Yeast Expression Vector

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  • METHOD:Using spinach to extract total RNA, the spinach GO cDNA was amplified by RT PCR. This cDNA was inserted into cloning vector pMD T and sequenced. The cDNA was then cloned into pPIC3 5K(P.Pastoris expression vector)by SnaBI/NotI double digestion.
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