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XUE Qiao-ru, LIANG Wei-yang, CHEN Yu-kun. Determination of peptide contents of hepatocyte growth-promoting factor for injection by acid hydrolytic method[J]. Journal of China Pharmaceutical University, 2011, 42(4): 333-336.
Citation: XUE Qiao-ru, LIANG Wei-yang, CHEN Yu-kun. Determination of peptide contents of hepatocyte growth-promoting factor for injection by acid hydrolytic method[J]. Journal of China Pharmaceutical University, 2011, 42(4): 333-336.
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Determination of peptide contents of hepatocyte growth-promoting factor for injection by acid hydrolytic method

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  • To investigate the analytic method for the determination of the peptide contents in hepatocyte growth-promoting factor for injection and compare the product quality differences in different factories.An AccQ-Tag method coupled with an HPLC using Kromasil 100-5 C18column (250 mm×4.6 mm，5 μm) was established for the determination of amino acids in acid hydrolytic samples.The contents of peptide was calculated by subtracting the amino acid amout in the non-hydrolytic samples from the total amino acids amount in the hydrolytic sample.The linearly dependent coefficient of each amino acid was in the range of 0.999 1-0.999 9（n=6）.The recove-ries of non-hydrolytic amino acids were 101.1%-104.6%，The recoveries of total amino acids were 101.8%-104.1%.The method is suitable for the determination of peptide contents in drug.
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