• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
SHEN Yan, LI Qian, FAN Da, LI Rui-jun, TU Jia-sheng. Influencing factors of hydrophobic modified hyaluronic acid-based cationic micelles and cell targeting in siRNA transfection[J]. Journal of China Pharmaceutical University, 2012, 43(2): 124-129.
Citation: SHEN Yan, LI Qian, FAN Da, LI Rui-jun, TU Jia-sheng. Influencing factors of hydrophobic modified hyaluronic acid-based cationic micelles and cell targeting in siRNA transfection[J]. Journal of China Pharmaceutical University, 2012, 43(2): 124-129.

Influencing factors of hydrophobic modified hyaluronic acid-based cationic micelles and cell targeting in siRNA transfection

More Information
  • The objective was to investigate the influencing factors on the siRNA delivery efficiency of hydrophobic modified hyaluronic acid with molecular weight 9 600 grafted with spermine and laurylamine cationic micelles(HAPL9600).The transfection efficiency of HAPL9600/siRNA was determined by flow cytometry.The influence of the molecular weight of HA,complexes forming time,the concentration of the HAPL9600,pH,the transfection medium and the weight ratio of HAPL9600 to siRNA on transfection were investigated.The competitive inhibition experiment was studied to investigate the in vivo cell target ability.The results indicated that HAPL9600 would reach the optimal transfection efficiency when the molecular weight of HA is 9 600 with 30 min of complexes forming time,15 mg/mL of concentration of the HAPL9600,8 of the weight ratio of HAPL9600 to siRNA at PBS(pH 6.8) transfection medium.The transfection efficiency of the HAPL9600 was reduced by competitive binding of free HA onto the CD44 receptors,thereby lowering the extent of cellular uptake via CD44 receptor mediated endocytosis,which suggested its in vitro targeting ability on the tumor cells.In all,HAPL9600 could obtain optimal,reduplicate and targeting transfection results by controlling related factors.
  • Related Articles

    [1]WANG Zhenghao, GAO Yafeng, ZHANG Lianjun, LIU Chang. Research progress of T cell anti-tumor function regulated by endoplasmic reticulum stress[J]. Journal of China Pharmaceutical University, 2022, 53(5): 518-524. DOI: 10.11665/j.issn.1000-5048.20220502
    [2]JIA Jian, WU Jianbing, ZHANG Yihua, HUANG Zhangjian. Synthesis and protective effect of carboxymethylcysteine L-arginate in bronchial epithelial cells[J]. Journal of China Pharmaceutical University, 2022, 53(2): 171-177. DOI: 10.11665/j.issn.1000-5048.20220206
    [3]CHEN Yingjie, GAO Xiangdong, CHEN Song. Effects and mechanisms of FGF21 on neuronal damage induced by rotenone[J]. Journal of China Pharmaceutical University, 2020, 51(6): 718-723. DOI: 10.11665/j.issn.1000-5048.20200611
    [4]SUN Yan, GAO Xiangdong, CHEN Song. Effect and mechanism of FGF21 on astrocyte damage induced by Aβ25-35[J]. Journal of China Pharmaceutical University, 2019, 50(4): 490-496. DOI: 10.11665/j.issn.1000-5048.20190415
    [5]CHEN Suting, CHEN Song, GAO Xiangdong. Construction, expression, purification and neuroprotective activity of TAT-FGF21 fusion protein[J]. Journal of China Pharmaceutical University, 2018, 49(4): 496-501. DOI: 10.11665/j.issn.1000-5048.20180417
    [6]CHEN Jianping, REN Xinsheng, SUN Zhonghua, GUO Zaiyu. Protective effects and mechanisms of breviscapine on endothelial cells[J]. Journal of China Pharmaceutical University, 2015, 46(5): 610-616. DOI: 10.11665/j.issn.1000-5048.20150516
    [7]ZHOU Huana, JIANG Nan, HAN Yuwei, KOU Junping. Advances of the traditional Chinese medicines modulating endoplasmic reticulum stress[J]. Journal of China Pharmaceutical University, 2015, 46(4): 385-392. DOI: 10.11665/j.issn.1000-5048.20150401
    [8]Effects of MCI-186 on Oxidative Stress-mediated Cell Damage Induced by H2O2 and Reactive Oxygen Species Formation in Brain Neurons[J]. Journal of China Pharmaceutical University, 2004, (6): 84-87.
    [9]WU Yu-Lin, YANG Zheng. Effects of MCI-186 on Oxidative Stress-mediated Cell Damage Induced by H_2O_2 and Reactive Oxygen Species Formation in Brain Neurons[J]. Journal of China Pharmaceutical University, 2004, (6).
    [10]Effects of Crocetin on the Myocardial Cell Damages Due to Oxidative Stress[J]. Journal of China Pharmaceutical University, 2003, (5): 66-69.

Catalog

    Article views (1971) PDF downloads (2117) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return