• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
HU Shan, DENG Yongchuan. Mechanism of isorhamnetin on breast cancer cells[J]. Journal of China Pharmaceutical University, 2013, 44(6): 563-567. DOI: 10.11665/j.issn.1000-5048.20130615
Citation: HU Shan, DENG Yongchuan. Mechanism of isorhamnetin on breast cancer cells[J]. Journal of China Pharmaceutical University, 2013, 44(6): 563-567. DOI: 10.11665/j.issn.1000-5048.20130615
shu

Mechanism of isorhamnetin on breast cancer cells

More Information
    Graphical Abstract
    • Abstract
  • This aim of this study is to explore the effect of isorhamnetin on the cell proliferation of breast cancer cells. Two breast cancer cell lines MCF7 and BT549 treated with different concentrations of isorhamnetin were examined for cell proliferation by using MTT assay. The effect of isorhamnetin on cell cycle and apoptosis was determined by flowcytometry. The effect of isorhamnetin on cell proliferation and apoptosis signaling pathway was examined by Western blot analysis. The results showed isorhamnetin can significantly inhibited the proliferation of breast cancer cell lines MCF7 and BT-549, and its IC50 was 11. 72 μmol/L and 8. 74 μmol/L, respectively. Isorhamnetin treatment resulted in cell cycle arrest at G1 phase and promote apoptosis. Consistent with this finding, isorhamnetin treatment significantly inhibited the AKT and ERK phosphorylation and the expression of proliferating nuclear antigen Ki67. Furthermore, isorhamnetin resulted in the decreased expression of Bcl-2 with concomitant decrease of Bcl-2 expression, subsequently causing the increase of cleaved Caspase-3 and cell apopotosis. Thus, isorhamnetin can significantly inhibit breast cancer cells by both inhibiting cell proliferation and promoting apoptosis.
    • FullText(HTML)
    • References (15)
  • [1]
    Wang ZR,Wang L,Yin HH.Effect of seabuckthorn flavone on myocardial contraction and calcium ion transportation of heart failure that induced by stretch[J].Space Med & Med Engineer(航天医学与医学工程),2000,13(1):6.
    [2]
    Zhao ZG,Liu YC.Cardiovascular protective effect of isorhanmetin[J].Med Recapitul(医学综述),2008,14(15):2 321-2 323.
    [3]
    Li Y,Wang PZ,Zhang YH.The inhibitory effect of isorhamnetin on growth of human gastric caricnoma cells[J].Chin Primary Health Care(中国初级卫生保健),2008,22(6):58-59.
    [4]
    Jihed B,Bhouri W,Ben Sghaier M,et al.Flavonoids products from Nitraria retusa leaves promote lymphoblastoid cells apoptosis[J].Nutr Cancer,2012,64(7):1 095-1 102.
    [5]
    Zhu L,Wang ZR,Zhou LM,et al.Effects and mechanisms of isorhamnetin on lung carcinoma[J].Space Med Med Eng(航天医学与医学工程),2005,18(5):381-383.
    [6]
    Li C,Yang X,Hu JB,et al.Isorhamnetin suppresses the growth of gefitinib resistant human lung cancer PC9 cells[J].Heral Med(医药导报),2012,31(7):831-834.
    [7]
    Luo HQ,Li XY,Guan CN,et al.Effect of isorhamnetin on the growth and proliferation of nasopharyngeal carcinoma cells[J].J Guangdong Med Coll(广东医学院学报),2011,29(2):119-121.
    [8]
    Teng BS,Lu YH,Wang ZT,et al.In vitro anti-tumor activity of isorhamnetin isolated from Hippophae rhamnoides L.against BEL-7402 cells[J].Pharmacol Res,2006,54(3):186-194.
    [9]
    Ma G,Yang C,Qu Y,et al.The flavonoid component isorhamnetin in vitro inhibits proliferation and induces apoptosis in Eca-109 cells[J].Chem Biol Interact,2007,167(2):153-160.
    [10]
    Jaramillo S,Lopez S,Varela LM,et al.The flavonol isorhamnetin exhibits cytotoxic effects on human colon cancer cells[J].J Agric Food Chem,2010,58(20):10 869-10 875.
    [11]
    Boubaker J,Ben Sghaier M,Skandrani I,et al.Isorhamnetin 3-O-robinobioside from Nitraria retusa leaves enhance antioxidant and antigenotoxic activity in human chronic myelogenous leukemia cell line K562[J].BMC Complement Altern Med,2012,12:135.
    [12]
    Ramachandran L,Manu KA,Shanmugam MK,et al.Isorhamnetin inhibits proliferation and invasion and induces apoptosis through the modulation of peroxisome proliferator-activated receptor γ activation pathway in gastric cancer[J].J Biol Chem,2012,287(45):38 028-38 040.
    [13]
    Kim JE,Lee DE,Lee KW,et al.Isorhamnetin suppresses skin cancer through direct inhibition of MEK1 and PI3-K[J].Cancer Prev Res,2011,4(4):582-591.
    [14]
    Lee HJ,Lee HJ,Lee EO,et al.Mitochondria-cytochrome C-caspase-9 cascade mediates isorhamnetin-induced apoptosis[J].Cancer Lett,2008,270(2):342-353.
    [15]
    Wu XJ,Wu KN.Antiproliferative effect of curcumin on human breast cancer of MCF-7 cells[J].Acta Acad Med Mil Tert(第三军医大学学报),2006,28(18):1 870-1 872.
    • Related Articles

  • Related Articles

    [1]LIU Mingxuan, GUO Yun, SUN Tao. Drug delivery systems for sensitization of glioblastoma radiotherapy[J]. Journal of China Pharmaceutical University, 2024, 55(5): 624-633. DOI: 10.11665/j.issn.1000-5048.2024050401
    [2]YE Zhenning, WU Zhenghong, ZHANG Huaqing. Research progress of blood-brain barrier crossing strategies and brain-targeted drug delivery mediated by nano-delivery system[J]. Journal of China Pharmaceutical University, 2024, 55(5): 590-602. DOI: 10.11665/j.issn.1000-5048.2024052202
    [3]LUO Xuelian, WU Chengsheng, ZHA Cheng, LIU Sheng. Research progress and prospects of implantable drug delivery systems for postoperative tumor therapy[J]. Journal of China Pharmaceutical University, 2024, 55(4): 538-547. DOI: 10.11665/j.issn.1000-5048.2024040901
    [4]WANG Shihao, LIU Lifeng, DING Yang, LI Suxin. Research progress of pH-responsive drug delivery systems in cancer immunotherapy[J]. Journal of China Pharmaceutical University, 2024, 55(4): 522-529. DOI: 10.11665/j.issn.1000-5048.2024011902
    [5]YU Jiayu, LIN Zezhi, CAO Wei, ZHANG Jianjun, WEI Yuanfeng, GAO Yuan, QIAN Shuai. Research progress of bio-metal organic frameworks in drug delivery system[J]. Journal of China Pharmaceutical University, 2023, 54(1): 23-33. DOI: 10.11665/j.issn.1000-5048.20221111003
    [6]LI Haoxian, LIN Huaqing, CHEN Jingwen, WANG Liyuan. Research progress of carbon nanomaterials in cancer drug delivery[J]. Journal of China Pharmaceutical University, 2019, 50(1): 100-106. DOI: 10.11665/j.issn.1000-5048.20190114
    [7]FAN Qianqian, XING Lei, QIAO Jianbin, ZHANG Chenglu, JIANG Hulin. Advances in drug delivery systems for the treatment of liver fibrosis[J]. Journal of China Pharmaceutical University, 2018, 49(3): 263-271. DOI: 10.11665/j.issn.1000-5048.20180302
    [8]HE Wei, QI Haixia, DONG Lei, ZHANG Junfeng. Research advances in drug delivery system targeting immune system[J]. Journal of China Pharmaceutical University, 2015, 46(5): 513-520. DOI: 10.11665/j.issn.1000-5048.20150501
    [9]WU Qu, HUO Meirong, ZHOU Jianping. Advances on novel drug delivery system[J]. Journal of China Pharmaceutical University, 2013, 44(2): 97-104. DOI: 10.11665/j.issn.1000-5048.20130201
    [10]TANG Yue, KE Xue. Advances of mesoporous silica nanoparticles as drug delivery system[J]. Journal of China Pharmaceutical University, 2012, 43(6): 567-572.

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：297223

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return