Anticomplementary effects of the extracts of five<i> Verbenaceae</i> herbs<i> in vitro</i>

JIAO Yang; ZOU Luhui; QIU Li; XIE Yunfeng; LUO Yan; XIE Jizhao

doi:10.11665/j.issn.1000-5048.20160413

Journal of China Pharmaceutical University > 2016 > 47(4): 469-473. > DOI: 10.11665/j.issn.1000-5048.20160413

JIAO Yang, ZOU Luhui, QIU Li, XIE Yunfeng, LUO Yan, XIE Jizhao. Anticomplementary effects of the extracts of five Verbenaceae herbs in vitro[J]. Journal of China Pharmaceutical University, 2016, 47(4): 469-473. DOI: 10.11665/j.issn.1000-5048.20160413

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Anticomplementary effects of the extracts of five Verbenaceae herbs in vitro

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The inhibition effects of the extracts of five Verbenaceae herbs, Clerodendrum fortunatum L. , Clerodendrum japonicum(Thunb. )Sweet, Clerodendrum philippinum Schauer var. simplex Moldenke, Callicarpa longissima(Hemsl. )Merr. and Pygmaeopremna herbacea, were investigated by cell hemolysis model in vitro on classical and alternative complement activation pathways. The water extracts of Pygmaeopremna herbacea, the water extract and the ethanol extract of Clerodendrum fortunatum L. , the water extract and the ethanol extract of Callicarpa longissima(Hemsl. )Merr. , the ethanol extracts of Clerodendrum japonicum(Thunb. )Sweet and Clerodendrum philippinum Schauer var. simplex Moldenke showed inhibition cell hemolysis effects on the classical pathway. Their CH₅₀ values were 0. 092±0. 008, 0. 074±0. 008, 0. 088±0. 006, 0. 134±0. 017, 0. 123±0. 010, 0. 380±0. 080, and 0. 200±0. 015 g/L, respectively. The water extracts of Pygmaeopremna herbacea and Callicarpa longissima(Hemsl. )Merr. and the ethanol extract of Clerodendrum fortunatum L. showed inhibition cell hemolysis effects on alternative pathway. Their AP₅₀ values were 0. 533±0. 033, 0. 758±0. 031, and 0. 362±0. 029 g/L, respectively. Five Verbenaceae herbs appear good anticomplementary effects in vitro. The ethanol extract of Clerodendrum fortunatum L. showed the best inhibitory activity.

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