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WANG Ying, CHEN Youhua, Qi Jin, YU boyang. Comparison of two new on-line methods on screening peroxynitrite scavengers in Radix Scrophulariae[J]. Journal of China Pharmaceutical University, 2016, 47(5): 560-565. DOI: 10.11665/j.issn.1000-5048.20160509
Citation: WANG Ying, CHEN Youhua, Qi Jin, YU boyang. Comparison of two new on-line methods on screening peroxynitrite scavengers in Radix Scrophulariae[J]. Journal of China Pharmaceutical University, 2016, 47(5): 560-565. DOI: 10.11665/j.issn.1000-5048.20160509
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Comparison of two new on-line methods on screening peroxynitrite scavengers in Radix Scrophulariae

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    • Abstract
  • Two simple and efficient methods have been developed for screening and identification of natural peroxynitrite scavengers in Radix Scrophulariae (RS). Method I was based on HPLC-DAD-(luminol-peroxynitrite)-CL techniques combined with Q-TOF MS/MS analysis, while method II was based on the pre-column reaction with peroxynitrite followed by HPLC separation with Q-TOF MS/MS analysis. Five active constituents, P1(decaffeoylacteoside), P9(eoside), I6(6″-O-feruloylharpagide), P11(cis-acteoside)and P13(angoroside), were found to possess potential peroxynitrite-scavenging activity by method I, while P9 and P13 were also screened by method II. Method I requires more complex apparatus, but has advantages on simple detection and high sensitivity. Method II requires simpler apparatus than method I, but with more tedious detection and lower sensitivity. However, the methods established above would provide new ways for rapid detection of natural peroxynitrite-scavenging compounds in RX complex matrices.
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    • References (16)
  • [1]
    Deiana M,Aruoma OI,Bianchi ML,et al.Inhibition of peroxynitrite dependent DNA base modification and tyrosine nitration by the extra virgin olive oil-derived antioxidant hydroxytyrosol[J].Free Radic Biol Med,1999,26(5/6):762-769.
    [2]
    Ahmad R,Rasheed Z,Ahsan H. Biochemical and cellular toxicology of peroxynitrite:implications in cell death and autoimmune phenomenon[J].Immunopharmacol Immunotoxicol,2009,31(3):388-396.
    [3]
    Levrand S,Vannay-Bouchiche C,Pesse B,et al.Peroxynitrite is a major trigger of cardiomyocyte apoptosis in vitro and in vivo[J].Free Radic Biol Med,2006,41(6):886-895.
    [4]
    Liaudet L,Rosenblatt-Velin N,Pacher P. Role of peroxynitrite in the cardiovascular dysfunction of septic shock[J].Curr Vasc Pharmacol,2013,11(2):196-207.
    [5]
    Ho SC,Tang YL,Lin SM,et al.Evaluation of peroxynitrite-scavenging capacities of several commonly used fresh spices[J].Food Chem,2010,119(3):1102-1107.
    [6]
    Shen K,Kiu JP,Yu BY.Research advances in the mechanisms of active components in Chinese materia medica against oxidative stress-induced neuronal apoptosis[J].J China Pharm Univ(中国药科大学学报),2015,46(5):532-540.
    [7]
    Kassim M,Mansor M,Kamalden TA,et al.Caffeic acid phenethyl ester(CAPE):scavenger of peroxynitrite in vitro and in sepsis models[J].Shock,2014,42(2):154-160.
    [8]
    Xu M,Chen X,Gu Y,et al.Baicalin can scavenge peroxynitrite and ameliorate endogenous peroxynitrite-mediated neurotoxicity in cerebral ischemia-reperfusion injury[J].J Ethnopharmacol,2013,150(1):116-124.
    [9]
    Li QF,Shi SL,Liu QR,et al.Anticancer effects of ginsenoside Rg1,cinnamic acid,and tanshinone IIA in osteosarcoma MG-63 cells:nuclear matrix downregulation and cytoplasmic trafficking of nucleophosmin[J].Int J Biochem Cell B,2008,40(9):1918-1929.
    [10]
    Huang JC,Li DJ,Diao JC,et al.A novel fluorescent method for determination of peroxynitrite using folic acid as a probe[J].Talanta,2007,72(4):1283-1287.
    [11]
    Alvarez S,Zaobornyj T,Actis-Goretta L,et al.Polyphenols and red wine as peroxynitrite scavengers:a chemiluminescent assay[J].Ann Ny Acad Sci,2002,957:271-273.
    [12]
    Van Dyke K,Ghareeb E,Van Dyke M,et al.Ultrasensitive peroxynitrite-based luminescence with L-012 as a screening system for antioxidative/antinitrating substances,e.g. Tylenol(R)(acetaminophen),4-OH tempol,quercetin and carboxy-PTIO[J].Luminescence,2007,22(4):267-274.
    [13]
    Kumar SS,Priyadarsini KI,Sainis KB. Inhibition of peroxynitrite-mediated reactions by vanillin[J].J Agr Food Chem,2004,52(1):139-145.
    [14]
    Beckman JS,Chen J,Ischiropoulos H,et al.Oxidative chemistry of peroxynitrite[J].Methods Enzymol,1994,233:229-240.
    [15]
    Jing J,Chan CO,Xu L,et al.Development of an in-line HPLC fingerprint ion-trap mass spectrometric method for identification and quality control of Radix Scrophulariae[J].J Pharm Biomed Anal,2011,56(4):830-835.
    [16]
    Wu QA,Yuan QA,Liu EH,et al.Fragmentation study of iridoid glycosides and phenylpropanoid glycosides in Radix Scrophulariae by rapid resolution liquid chromatography with diode-array detection and electrospray ionization time-of-flight mass spectrometry[J].Biomed Chromatogr,2010,24(8):808-819.
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