Mechanism of lncRNA CTD-3252C9.4 on invasion and migration of pancreatic cancer Panc-1 cells<i> in vitro</i>

YIN Xin; SHI Wanyue; PAN Yi; JIN Liang

doi:10.11665/j.issn.1000-5048.20190215

Journal of China Pharmaceutical University > 2019 > 50(2): 230-237. > DOI: 10.11665/j.issn.1000-5048.20190215

YIN Xin, SHI Wanyue, PAN Yi, JIN Liang. Mechanism of lncRNA CTD-3252C9.4 on invasion and migration of pancreatic cancer Panc-1 cells in vitro[J]. Journal of China Pharmaceutical University, 2019, 50(2): 230-237. DOI: 10.11665/j.issn.1000-5048.20190215

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Mechanism of lncRNA CTD-3252C9.4 on invasion and migration of pancreatic cancer Panc-1 cells in vitro

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Abstract

The aim of this study is to investigate the inhibitory effect of long non-coding RNA(lncRNA)CTD-3252C9. 4 on migration and invasion of human pancreatic cancer cell Panc-1 in vitro and its mechanism. Panc-1 cells were stimulated by epidermal growth factor(EGF)in three-dimensional semi-solid system of cultured pancreatic cancer spheres. RT-qPCR was used to detected the transfection efficiency of lncRNA CTD-3252C9. 4. The effects of lncRNA CTD-3252C9. 4 and bone morphogenetix protein 7(BMP7)on the invasion and migration of Panc-1 cells were detected by scratch healing method and Transwell chamber method. The changes of target gene BMP7 and epithelial-mesenchymal transition(EMT)related proteins were verified by Western blot. EGF could significantly inhibit the expression of lncRNA CTD-3252C9. 4 in Panc-1 cells. The lncRNA can affect cells invasion and migration by inhibiting the transcription of the oncogene BMP7, then inhibit the process of EMT of tumors.
- lncRNA,
- bone morphogenetix protein 7,
- pancreatic cancer,
- invasion and metastasis,
- epithelial-mesenchymal transition

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Mechanism of lncRNA CTD-3252C9.4 on invasion and migration of pancreatic cancer Panc-1 cells in vitro

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