Ameliorative effect of psoralen on acute liver injury induced by carbon tetrachloride in mice

ZHANG Yan; JIANG Zhenzhou; ZHANG Luyong

doi:10.11665/j.issn.1000-5048.20210512

Journal of China Pharmaceutical University > 2021 > 52(5): 596-602. > DOI: 10.11665/j.issn.1000-5048.20210512

ZHANG Yan, JIANG Zhenzhou, ZHANG Luyong. Ameliorative effect of psoralen on acute liver injury induced by carbon tetrachloride in mice[J]. Journal of China Pharmaceutical University, 2021, 52(5): 596-602. DOI: 10.11665/j.issn.1000-5048.20210512

Citation:

PDF (10736 KB)

Ameliorative effect of psoralen on acute liver injury induced by carbon tetrachloride in mice

1.
New Drug Screening Center, Institute of Pharmaceutical Sciences, China Pharmaceutical University, Nanjing 210009
2.
Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou 510006, China

Funds: This study was supported by the Non-profit Industry Scientific Research Project (No.201507004-002) and China National Key Hi-Tech Innovation Project for the R&D of Novel Drugs (No.2015ZX09501004-002-004)

More Information

Received Date: March 01, 2021
Revised Date: September 16, 2021

Graphical Abstract

Abstract

Abstract

To investigate the ameliorative effect of psoralen (PSO) on carbon tetrachloride (CCl₄)-induced acute liver injury in mice and its potential mechanism, female C57BL/6J mice aged 6-8 weeks were continuously administrated with psoralen or positive control drug diallyl sulfide (DAS) intragastrically for 4 days.On day 4, except that the control group were treated with vehicle control, other groups were all given carbon tetrachloride intraperitoneally to establish a carbon tetrachloride acute liver injury model.Serum biochemical indicators alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were detected; liver pathological changes were observed by HE staining; cytochrome P450 2E1 (CYP2E1) protein levels were detected by Western blot; the protein level of CYP2E1 were detected by immunohistochemistry (IHC) staining; and the gene levels of CYP2E1, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by RT-PCR.Compared with the model group, psoralen could improve the inflammatory cell infiltration and hepatocyte necrosis caused by carbon tetrachloride, significantly reducing the serum ALT and AST levels, down-regulating the inflammatory factors TNF-α and IL-6 levels, and inhibiting CYP2E1 protein expression.The results show that psoralen can ameliorate the acute liver injury induced by carbon tetrachloride in mice, with the possible mechanism inhibiting the protein expression of CYP2E1.
- psoralen,
- carbon tetrachloride,
- acute liver injury,
- cytochrome P450 2E1

FullText(HTML)

References (22)

References

[1]	.Am J Chin Med，2016，44（1）：35-60.
[2]	Lu YQ，Zhang X，Wang JJ，et al.Research progress on chemical constituents and pharmacological actions of Psoralea Fructus［J］.Chin J Exp Tradit Med Form（中国实验方剂学杂志），2019，25（3）：180-189.
[3]	Wang LY，Su QB，Bi HE，et al.In vivo absorption of psoralen using improved rat intestinal perfusion model［J］.J China Pharm Univ（中国药科大学学报），2007，38（3）：265-268.
[4]	Lei Z，Ma WD，Yu KQ，et al.Pharmacokinetics research advances of coumarin compounds in vivo［J］.Chin Pharm（中国药业），2016，25（23）：5-8.
[5]	Liu XD，Wen WJ，Zhao ZJ. Protective effect of total flavonoids from Lithocarpus polystachyus rehd on CCl₄-induced acute liver injury in mice［J］.Her Med（医药导报），2020，39（12）：1626-1630.
[6]	Niu SL，Zhang XN，Wu ZL，et al. Hepatoprotective effect of total flavones of Artemisia capillaris thunb on acute hepatic injury in rats［J］.Her Med（医药导报），2016，35（3）：246-248.
[7]	Jin Y，Xu F，Lu Y. Discussion and suggestion on the method of histopathologic diagnosis of rat and mouse liver acute injury induced by carbon tetrachloride［J］.Chin J New Drugs（中国新药杂志），2014，23（19）：2224-2227.
[8]	Correia MA，Kwon D.Why Hepatic CYP2E1-elevation by itself is insufficient for inciting NAFLD/NASH：inferences from two genetic knockout mouse models［J］.Biology，2020，9（12）：419.
[9]	Zhou L，Tang J，Yang X，et al.Five constituents in Psoralea corylifolia L.attenuate palmitic acid-induced hepatocyte injury via inhibiting the protein kinase C-α/nicotinamide-adenine dinucleotide phosphate oxidase pathway［J］.Front Pharmacol，2019，10 ：1589.
[10]	Wang XM，Peng PK，Pan ZQ，et al. Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells［J］.Biol Res，2019，52（1）：34.
[11]	Li DG，Wang JH，Gao SF，et al.Effects of psoralen on the proliferation and oxidative stress of hepatic stellate cell（HSC-T6） and the secretion of collagen Ⅰ［J］.Nat Prod Res Dev（天然产物研究与开发），2011，23（1）：35-38.
[12]	Yang K，Gao R，Ma YZ，et al.Research progress on mechanism of pharmacological effects and hepatotoxicity of psoralen［J］.Chin Tradit Herbal Drugs（中草药），2021，52（1）：289-298.
[13]	Xu B，Tian J，Ma N，et al. Progress in animal models of liver injury［J］.China Mod Med（中国当代医药），2019，26（14）：38-40，44.
[14]	Peng XY，Dai CS，Liu QW，et al. Curcumin attenuates on carbon tetrachloride-induced acute liver injury in mice via modulation of the Nrf2/HO-1 and TGF-β1/Smad3 pathway［J］.Molecules，2018，23（1）：215.
[15]	Ta?eb D，Malicet C，Garcia S，et al. Inactivation of stress protein p8 increases murine carbon tetrachloride hepatotoxicity via preserved CYP2E1 activity［J］.Hepatology，2005，42（1）：176-182.
[16]	Liang PS，Zhou W，Jiang ZZ，et al. Mechanism of psoralen in aggravating hepatotoxicity induced by CCl₄ by delaying liver regeneration［J］.China J Chin Mater Med（中国中药杂志），2020，45（12）：2916-2923.
[17]	Guo FC，Zhuang XY，Han MY，et al. Polysaccharides from Enteromorpha prolifera protect against carbon tetrachloride-induced acute liver injury in mice via activation of Nrf2/HO-1 signaling，and suppression of oxidative stress，inflammation and apoptosis［J］.Food Funct，2020，11（5）：4485-4498.
[18]	Zeng BY，Su MH，Chen QX，et al. Protective effect of a polysaccharide from Anoectochilus roxburghii against carbon tetrachloride-induced acute liver injury in mice［J］.J Ethnopharmacol，2017，200：124-135.
[19]	Feng L，Wang L，Jiang XH. Pharmacokinetics，tissue distribution and excretion of coumarin components from Psoralea corylifolia L. in rats［J］. Arch Pharm Res，2010，33（2）：225-230.
[20]	Wang X，Lou YJ，Wang MX，et al. Furocoumarins affect hepatic cytochrome P450 and renal organic ion transporters in mice［J］.Toxicol Lett，2012，209（1）：67-77.
[21]	Yang CS，Chhabra SK，Hong JY，et al. Mechanisms of inhibition of chemical toxicity and carcinogenesis by diallyl sulfide（DAS） and related compounds from garlic［J］.J Nutr，2001，131（3s）：1041S-1045S.
[22]	Brady JF，Ishizaki H，Fukuto JM，et al. Inhibition of cytochrome P-450 2E1 by diallyl sulfide and its metabolites［J］.Chem Res Toxicol，1991，4（6）：642-647.

[1]	XU Linhao, LIU Xinze, LIU Wei, XIANG Ping, HANG Taijun, YANG Shuo, YAN Hui. Application of zebrafish model in the metabolism of new psychoactive substances[J]. Journal of China Pharmaceutical University, 2022, 53(5): 606-612. DOI: 10.11665/j.issn.1000-5048.20220511
[2]	CHENG Liyuan, LIU Wei, GUO Xiaoyan, GE Xiaoai, WANG Dingding, WANG Tao. Effects and mechanism of zidovudine on glucolipid metabolic balance in mice[J]. Journal of China Pharmaceutical University, 2022, 53(3): 340-347. DOI: 10.11665/j.issn.1000-5048.20220312
[3]	WANG Jingqi, WANG Ye, ZHANG Yi, LIANG Juncheng, DENG Yanping, HANG Taijun, SONG Min. Metabolomic study on clinical staging of methamphetamine detoxification by LC-QTOF-MS[J]. Journal of China Pharmaceutical University, 2022, 53(3): 314-322. DOI: 10.11665/j.issn.1000-5048.20220309
[4]	HE Mingzhe, PENG Ying, WANG Guangji, A Jiye, ZHENG Yiwen, SUN Jianguo. Mechanism of sodium salicylate-induced damage to HEI-OC1 cells based on metabonomics[J]. Journal of China Pharmaceutical University, 2021, 52(5): 566-572. DOI: 10.11665/j.issn.1000-5048.20210508
[5]	WU Yubing, YIN Lifang, QIN Chao. Clinical application and detection of matrix metalloproteinases in diagnosis[J]. Journal of China Pharmaceutical University, 2020, 51(5): 614-621. DOI: 10.11665/j.issn.1000-5048.20200514
[6]	TAO Yingjun, WU Jie, LIU Chang. Application of proteomics in diabetes and its complications[J]. Journal of China Pharmaceutical University, 2020, 51(3): 368-373. DOI: 10.11665/j.issn.1000-5048.20200315
[7]	YIN Kunpeng, ZHENG Hao, XIE Binxin, LI Ping, ZHANG Lei, FAN Yong, ZHU Wei, QI Lianwen. Clinical metabolomics in diagnosis and therapy of coronary artery disease[J]. Journal of China Pharmaceutical University, 2017, 48(6): 629-634. DOI: 10.11665/j.issn.1000-5048.20170601
[8]	ZHANG Xue, WANG Yuhao, ZHENG Yunsi, HE Hua, LIU Xiaoquan. Evaluation of the protective effect of salvianolic acid A on ischemic heart failure by a multi-target pharmacokinetic-pharmacodynamic model[J]. Journal of China Pharmaceutical University, 2016, 47(5): 587-594. DOI: 10.11665/j.issn.1000-5048.20160514
[9]	LI Chen, YUAN Linhua, MA Xueqin, LIU Xiaoquan, XI Tao. Variation of cardiovascular risk biomarkers in type 2 diabetes mellitus[J]. Journal of China Pharmaceutical University, 2013, 44(3): 257-262. DOI: 10.11665/j.issn.1000-5048.20130314
[10]	HUANG Yin, XU Fengguo, ZHANG Wei, ONG Choonnam, ZHANG Zunjian. Progress for pharmacometabolomics and its applications[J]. Journal of China Pharmaceutical University, 2013, 44(2): 105-112. DOI: 10.11665/j.issn.1000-5048.20130202

Cited By

Get Citation

PDF

XML

Article views (130) PDF downloads (424)

Turn off MathJax

Article Contents

Abstract

References

Ameliorative effect of psoralen on acute liver injury induced by carbon tetrachloride in mice

Abstract

References

Related Articles

Catalog

Ameliorative effect of psoralen on acute liver injury induced by carbon tetrachloride in mice

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content