rhIL23R-CHR/Fc fusion protein inhibits Act-HaCaT inflammation and proliferation by downregulating ENST00000522718

WANG Liming; JIANG Xiaomeng; GAO Yue; MA Yuxiao; ZENG Aizhong; GUO Wei

doi:10.11665/j.issn.1000-5048.20220613

Journal of China Pharmaceutical University > 2022 > 53(6): 734-741. > DOI: 10.11665/j.issn.1000-5048.20220613

WANG Liming, JIANG Xiaomeng, GAO Yue, MA Yuxiao, ZENG Aizhong, GUO Wei. rhIL23R-CHR/Fc fusion protein inhibits Act-HaCaT inflammation and proliferation by downregulating ENST00000522718[J]. Journal of China Pharmaceutical University, 2022, 53(6): 734-741. DOI: 10.11665/j.issn.1000-5048.20220613

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rhIL23R-CHR/Fc fusion protein inhibits Act-HaCaT inflammation and proliferation by downregulating ENST00000522718

1.
School of Life Science and Technology, China Pharmaceutical University,Nanjing 211198
2.
Department of Gastroenterology, Sir Run Run Hospital,Nanjing Medical University,Nanjing 211112, China

Funds: This study was supported by the National Natural Science Foundation of China (No.81973221); and the Chinese Foundation for Hepatitis Prevention and Control——TianQing Liver Disease Research Fund Project (No.TQGB20210089)

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Received Date: September 22, 2022
Revised Date: November 03, 2022

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Abstract

Abstract

Psoriasis is an autoimmune disease characterized by chronic skin inflammation, and its etiology and pathogenesis have not been fully elucidated to date. In the previous study, rhIL23R-CHR/Fc fusion protein had been found to significantly relieve the symptoms of psoriasis mice and the pharmacological mechanism had been initially elucidated.In this study, we established a psoriasis cell model (Act-HaCaT) using TNF-α-activated human immortalized keratinocytes (HaCat).In our current study, the lncRNA that plays a key role in the regulation of Act-HaCaT function by the rhIL23R-CHR/Fc fusion protein was screened by transcriptome sequencing combined with qRT-PCR.The results showed that rhIL23R-CHR/Fc fusion protein significantly inhibited cell proliferation and inflammatory factor production in Act-HaCaT.lncRNA ENST00000522718 was obtained by screening, and knockdown of ENST00000522718 was found to significantly inhibit cell proliferation and inflammatory factor production.Our findings suggest that ENST00000522718 plays an important role in the pathological mechanism of psoriasis.
- lncRNA,
- rhIL23R-CHR/Fc fusion protein,
- proliferation,
- inflammatory factor

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References (35)

References

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rhIL23R-CHR/Fc fusion protein inhibits Act-HaCaT inflammation and proliferation by downregulating ENST00000522718

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