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DU Lixia, LI Dian, ZHUO Huirong, SHI Mengnan, CHEN Yanning, LI Lanfang, HOU Shaoyang. Primer probes for targeted identification and detection of Bifidobacterium animalis and their application[J]. Journal of China Pharmaceutical University, 2023, 54(2): 255-262. DOI: 10.11665/j.issn.1000-5048.20220912001
Citation: DU Lixia, LI Dian, ZHUO Huirong, SHI Mengnan, CHEN Yanning, LI Lanfang, HOU Shaoyang. Primer probes for targeted identification and detection of Bifidobacterium animalis and their application[J]. Journal of China Pharmaceutical University, 2023, 54(2): 255-262. DOI: 10.11665/j.issn.1000-5048.20220912001
shu

Primer probes for targeted identification and detection of Bifidobacterium animalis and their application

  • College of Pharmacy, Heze University, Heze 274015, China

Funds: This study was supported by the Natural Science Foundation of Shandong Province (No.ZR2022QB146); and the Heze University PhD Fund Planning (No.XY21BS35)
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  • Received Date: September 11, 2022
  • Revised Date: March 26, 2023
    Graphical Abstract
    • Abstract
  • In this study, in order to overcome the shortcomings of the current methods used to identify Bifidobacterium animalis, such as long time, complicated operation and low adaptability of experimental environment, specific primer probes were designed based on ERIC-PCR technology to identify and detect B.animalis.Based on the genomic DNA of B.animalis HP-B1124, the ERIC-PCR reaction conditions of B.animalis HP-B1124 were optimized, and the ERIC-PCR fragments were obtained one by one and sequenced.Two pairs of specific primer probes were designed.The accuracy, specificity, limitation and universality of the two pairs of primer probes were evaluated, and the two pairs of specific primer probes were used for testing the products containing B.animalis in the commercially published formula.The two pairs of specific primer probes designed in this study could be used for identified strains of B.animalis more simply, quickly and targeted.This method has optimized the current relatively traditional methods of pure culture and plate counting identification of B.animalis, and has solved the high requirements of SNP genotyping technology and real-time fluorescence quantitative PCR method for experimental equipment and reagents in the identification of B.animalis to a certain extent.It has the characteristics of low cost, high specificity and earn a broad market development prospect.
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    • References (22)
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