Progresses of mass spectrometry-based analysis of <i>N</i>-glycoproteins

TIAN Zhixin

doi:10.11665/j.issn.1000-5048.2023062701

Journal of China Pharmaceutical University > 2023 > 54(6): 662-673. > DOI: 10.11665/j.issn.1000-5048.2023062701

TIAN Zhixin. Progresses of mass spectrometry-based analysis of N-glycoproteins[J]. Journal of China Pharmaceutical University, 2023, 54(6): 662-673. DOI: 10.11665/j.issn.1000-5048.2023062701

Citation:

TIAN Zhixin. Progresses of mass spectrometry-based analysis of N-glycoproteins[J]. Journal of China Pharmaceutical University, 2023, 54(6): 662-673. DOI: 10.11665/j.issn.1000-5048.2023062701

Citation:

TIAN Zhixin. Progresses of mass spectrometry-based analysis of N-glycoproteins[J]. Journal of China Pharmaceutical University, 2023, 54(6): 662-673. DOI: 10.11665/j.issn.1000-5048.2023062701

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Progresses of mass spectrometry-based analysis of N-glycoproteins

TIAN Zhixin

School of Chemical Science and Engineering, TongJi University, Shanghai 200092, China

Funds: This study was supported by the National Natural Science Foundation of China (No.22074105, No.21775110, No.21575104)

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Received Date: June 26, 2023
Revised Date: December 10, 2023

Graphical Abstract

Abstract

Abstract

N-linked glycosylation is a common post-translational modification on proteins, which exhibits the same macro-heterogeneity of modification site as other small molecule modifications (such as methylation, acetylation, phosphorylation), i.e., the amino acid sequence of a protein has multiple putative modification sites. However, compared to small molecule modifications with single structures, N-glycosylation modification have tens of thousands of structures from multiple structural dimensions such as different monosaccharide compositions, sequence structures, linking structures, isomerism, and three-dimensional conformation.This results in additional micro-heterogeneity of modification site of N-glycosylation, i.e., the same N-glycosylation site can be modified with different glycans with a certain stoichiometric ratio.N-glycosylation modification regulates the structure and function of N-glycoproteins in a site- and structure-specific manner, and differential expression of N-glycosylation under disease conditions needs to be characterized through site- and structure-specific quantitative analysis.This article mainly introduces the latest development of mass spectrometry-based site- and structure-specific quantitative N-glycoproteomics and its applications in biomedical fields.
- N-glycoprotein,
- glycosylation sites,
- glycan structure,
- qualitative,
- quantitative

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References (48)

References

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