Analysis of chemical constituents of the effective part of anti-EGFR of <i>Lonicera macranthoides </i>Hand.-Mazz by UPLC-Q-TOF-MS

REN Weiguang; LIN Sensen; LI Wentao; HUANG Linfang

doi:10.11665/j.issn.1000-5048.20130607

Journal of China Pharmaceutical University > 2013 > 44(6): 526-530. > DOI: 10.11665/j.issn.1000-5048.20130607

REN Weiguang, LIN Sensen, LI Wentao, HUANG Linfang. Analysis of chemical constituents of the effective part of anti-EGFR of Lonicera macranthoides Hand.-Mazz by UPLC-Q-TOF-MS[J]. Journal of China Pharmaceutical University, 2013, 44(6): 526-530. DOI: 10.11665/j.issn.1000-5048.20130607

Citation:

PDF (1124 KB)

Analysis of chemical constituents of the effective part of anti-EGFR of Lonicera macranthoides Hand.-Mazz by UPLC-Q-TOF-MS

More Information

Graphical Abstract

Abstract

Abstract

Application of high-throughput drug screening, screening anti-epidermal growth factor receptor(EGFR)activity of Chinese herb extracts, and the constituents in Lonicera macranthoides Hand. -Mazz were determined by UPLC-Q-TOF/MS. Herbs were percolated through petroleum ether, ethanol extract, ethyl acetate extraction and boiling water, obtaining four corresponding parts. The activity of EGFR was detected based on HTRF. The variation of fluorescence signal and signal value at 620 nm and 665 nm were determined; the inhibition rate of extracts were calculated. Chromatographic separation was performed on a C₁₈ column(2. 1 mm×50 mm, 1. 7 μm)with a gradient elution of 0. 1% formic acid aqueous solution- acetonitrile. The mass spectrometer equipped with electrospray ionization source was used as defector and data were collected under the positive ion mode. Strong inhibitory activity of EGFR was found for the ethyl acetate part of L. macranthoides Hand. -Mazz, with IC₅₀ value of 2. 027 μg/mL. The information of positive ion and the relative molecular mass were distinguished by Q-TOF/MS, which identified the 7 corresponding parts of L. macranthoides Hand. -Mazz. : caffeic acid, esculetin, quercetin, 3, 5- O-dicaf feoylquinic acid, hinoki flavone, chlorogenic acid and β-sitosterol as well as 4 unknown substances. 3, 5-O-dicaf feoylquinic acid, caffeic acid and hinoki flavone are the main components. The results showed that L. macranthoides Hand. -Mazz contained phenolic acids and flavonoids inhibiting EGFR, which provided the basis for the research and development of antitumor drugs in the future.
- EFGR kinase inhibitors,
- high-throughput drug screening,
- Lonicera macranthoides,
- extract,
- active ingredients,
- UPLC-Q-TOF/MS

FullText(HTML)

References (21)

References

[1]	Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia:Part 1(中华人民共和国药典:一部)[M].Beijing:China Medical Science Press,2010:28.
[2]	Chen Y,Zhao XZ,Jia XD,et al.Progress in study of chemical consti-anti-tumor activities of Lonicera macranthoides Hand-Mazz[C].Teig Nation Nat Med Res Acad Sem(全国第八届天然药物资源学术研讨会论文集).Nanjing:2008.
[3]	Li SY, Zheng X, Liao DF. Advances in the study of Flos Lonicerae Confusae[J].J Univ South China(Med Edit)[南华大学学报(医学版)],2009,37(6):744-746.
[4]	Chen J,Xu XF,Cai XY,et al.Chemical constituents in the buds of Lonicera macranthoides[J].Chin J Nat Med(中国天然药物),2006,5(4):347-351.
[5]	Chen Y,Feng X,Wang M,et al.Triterpene glycosides from Lonicera Li isolation and structural determination of glycosides from flower buds of Lonicera macranthoides[J].Chem Nat Compd,2009,45(4):514-518.
[6]	Zhang XN,Tang Q,Hou M,et al.Comparison of the pharmacological effects between L.macranthoides Hand.-Mazz and Lonicera japonica Thunb[J].Chin Pharmacol Bull(中国药理学通报),2012,28(11):1 582-1 585.
[7]	Zeng S.Research progresses of epidermal growth factor receptor tyrosine kinase inhibitors[J].Mod Med Health(现代医药卫生),2010,26(20):3 103-3 015.
[8]	Zhuang HQ,Yuan ZY,Wang J,et al.Research progress on criteria for discontinuation of EGFR inhibitor therapy[J].A Peer-Rev B Sci,2012,12(5):263-270.
[9]	Zhang XY,Xu LY,Wang H.The relationship between EGFR mutations and response and prognosis of tyrosine kinase inhibitors in advanced non-small-cell lung cancer[J].Chin J Lung Cancer(中国肺癌杂志),2008,11(2):206-213.
[10]	Zhang SJ.Antioxidant activity constituents of stems and leaves of Lonicera japonica Thunb(忍冬茎叶抗氧化活性成分研究)[D].Xianyang:Chinese North West Agriculture Forestry University,2012.
[11]	Lu XL,Qiao Y,Zhang XM,et al.Chemical constituents from Ceratophyllum demersum[J].Acta Bot Yunnanica(云南植物研究),2007,29(2):26-31.
[12]	Zhu XF,Zhang HX,Raymond L.Phenolic compounds from the leaf extract of Artichoke(Cynara scolymus L.)and their antimicrobial activities[J].J Agric Food Chem 2004,52(24):7 272-7 278.
[13]	Romani C,Galardi P,Pinelli,et al.Inhibition of tumor growth by quercetin with increase of survival and prevention of cachexia in Walker 256 rats[J].Biochem Biophys Res Commun,2002,56(7/8):469-474.
[14]	Normanno N,Maiello MR,De LA.Target-based agents against ErbB receptors and their ligands:a novel approach to cancer treatment[J].J Cell Phy,2003,194(1):13-19.
[15]	Camila AC,Maria EF,Rodrigo A.et al.Inhibition of tumor growth by quercetin with increase of survival and prevention of cachexia in Walker 256 tumor-bearing rats[J].Biochem Biophys Res Commun,2011,406(4):638-642.
[16]	Wang L,Wang B,Lia H,et al.Role of quercetin in vascular physiology[J].Int J Pharm,2012,690(1/2/3):133-141.
[17]	Yuki S,Shirou I,Toshimitsu K,et al.In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid[J].Int J Pharm,2011,403(1/2):136-138.
[18]	Chai DW.Screening of the natural anti-tumor substances in fruits of Lonicera maackii(Rupr.)(金银忍冬果实抗肿瘤物质的筛选)[D].Lanzhou:Chinese Lanzhou University,2010.
[19]	Ma MF,Chen J,Shi Y,et al.Pharmacological activities of biflavonoids from Fokienia[J].Tibet Sci Technol(西藏科技),2012,231(6):65-66.
[20]	Huang J,Yang GY,Li HJ,et al.Progress in mechanism studies of antioxidants[J].Chin Nature(自然杂志),2004,26(2):74-78.
[21]	Wang Y,Xiao CY,Tian L,et al.Emerging varieties of Flos Lonicerae——Lonicera macranthoides Hand.-Mazz[C].Luo-yang:Ten Nat Chin Med Nat l med Acad Sem(十届全国中药和天然药物学术研讨会),2009.

[1]	LOU Fangning, ZHENG Mingyue, CHEN Kaixian, ZHANG Sulin. Research progress of cGAS-STING signaling pathway modulators in immunotherapy[J]. Journal of China Pharmaceutical University, 2024, 55(1): 15-25. DOI: 10.11665/j.issn.1000-5048.2023112402
[2]	WU Congyu, ZHOU Yue, SHANGGUAN Luxi, YANG Yani, WANG Jingya, YU Junhe, GONG Shuaishuai, KOU Junping. Progress of research on the pharmacological effects and mechanisms of emodin[J]. Journal of China Pharmaceutical University, 2023, 54(5): 634-643. DOI: 10.11665/j.issn.1000-5048.2023052501
[3]	ZHANG Yuxin, DING Ming, LIU Jun. Research progress of proximity labeling technology based on biotin ligase in proteomics[J]. Journal of China Pharmaceutical University, 2022, 53(1): 18-24. DOI: 10.11665/j.issn.1000-5048.20220103
[4]	LIU Yuhong, ZHANG Fangfang, LIU Jianxing, LIU Yue, YANG Yue, JIN Liang. Interplay between non-coding RNA and insulin signaling pathway and its clinical application[J]. Journal of China Pharmaceutical University, 2021, 52(3): 279-286. DOI: 10.11665/j.issn.1000-5048.20210303
[5]	TANG Keqin, LIN Huaqing, LI Shuhong, DONG Lixin, LU Bohong, JIANG Hong. Advances in tumor targeted nanocrystals[J]. Journal of China Pharmaceutical University, 2020, 51(4): 418-424. DOI: 10.11665/j.issn.1000-5048.20200405
[6]	LIU Li, ZHANG Qianwen, NONG Cheng, ZHANG Xi, XU Xiaoting, Mohammed Ismail, XIAO Li, JIANG Zhenzhou, ZHANG Luyong, SUN Lixin. Research progress of lncRNA regulating signal transduction pathway in liver diseases[J]. Journal of China Pharmaceutical University, 2020, 51(3): 277-286. DOI: 10.11665/j.issn.1000-5048.20200304
[7]	MEI Jiahao, HONG Ze, WANG Chen. Advances of drugs in targeting cGAS-STING signaling pathway[J]. Journal of China Pharmaceutical University, 2020, 51(3): 249-259. DOI: 10.11665/j.issn.1000-5048.20200301
[8]	XU Han, ZHANG Yuanyuan, KOU Junping. Advances in drug-induced gastrointestinal bleeding and its pathological mechanism[J]. Journal of China Pharmaceutical University, 2019, 50(6): 659-665. DOI: 10.11665/j.issn.1000-5048.20190604
[9]	WANG Xue, ZHANG Pinghu. Advances in research on the modulation of autophagy by Ras/Raf/MEK/ERK signaling pathway[J]. Journal of China Pharmaceutical University, 2017, 48(1): 110-116. DOI: 10.11665/j.issn.1000-5048.20170117
[10]	HUANG Shaoliang, ZHAO Li, GUO Qinglong, WU Yulin. Advances of Hedgehog pathway in tumor resistance[J]. Journal of China Pharmaceutical University, 2016, 47(3): 259-266. DOI: 10.11665/j.issn.1000-5048.20160302

Cited By

Get Citation

PDF

XML

Article views (1526) PDF downloads (1776)

Turn off MathJax

Article Contents

Abstract

References

Analysis of chemical constituents of the effective part of anti-EGFR of Lonicera macranthoides Hand.-Mazz by UPLC-Q-TOF-MS

Abstract

References

Related Articles

Catalog

Analysis of chemical constituents of the effective part of anti-EGFR of Lonicera macranthoides Hand.-Mazz by UPLC-Q-TOF-MS

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content