• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
ZHOU Xingchun, LI Yingxin, HONG Wen, WANG Ying, YE Boping. Effect of Maculosin on fibrotic gene expression in lung fibroblasts[J]. Journal of China Pharmaceutical University, 2014, 45(4): 491-495. DOI: 10.11665/j.issn.1000-5048.20140419
Citation: ZHOU Xingchun, LI Yingxin, HONG Wen, WANG Ying, YE Boping. Effect of Maculosin on fibrotic gene expression in lung fibroblasts[J]. Journal of China Pharmaceutical University, 2014, 45(4): 491-495. DOI: 10.11665/j.issn.1000-5048.20140419
shu

Effect of Maculosin on fibrotic gene expression in lung fibroblasts

More Information
    Graphical Abstract
    • Abstract
  • Maculosin is a kind of cyclic dipeptides resulting from double condensations between L-proline and L-tyrosine. It was purified from the fermentation broth of strain CGD12, an Aspergillus fungi isolated from Rhizophora stylosa rhizosphere soil in the previous study. In the present study, lactate dehydrogenase(LDH)assay was applied to detect the cytotoxicity of Maculosin in lung fibroblasts. Real-time quantitative RT-PCR was used to assess its effects on the fibrotic-related gene expression. The results showed that Maculosin inhibits the activation of lung fibroblasts and attenuates the accumulation of extracellular matrix(ECM)induced by exogenous TGF-β2. Further study suggested that the anti-fibrotic activity of Maculosin may be related to repressing the expression of inflammatory cytokines and promoting the process of ECM degradation.
    • FullText(HTML)
    • References (15)
  • [1]
    Martins MB,Carvalho I.Diketopiperazines:biological activity and synthesis[J].Tetrahedron,2007,63(40):9 923-9 932.
    [2]
    Borthwick AD.2,5-Diketopiperazines:synthesis,reactions,medicinal chemistry,and bioactive natural products[J].Chem Rev,2012,112(7):3 641-3 716.
    [3]
    Wang Y,Wang P,Ma HG,et al.Developments around the bioactive diketopiperazines:a patent review[J].Expert Opin Ther Pat,2013,23(11):1 415-1 433.
    [4]
    Stierle AC,Cardellina JH,Strobel GA.Maculosin,a host-specific phytotoxin for spotted knapweed from Alternaria alternata[J].Proc Natl Acad Sci U S A,1988,85(21):8 008-8 011.
    [5]
    Strobel G,Kenfield D,Bunkers G,et al.Phytotoxins as potential herbicides[J].Experientia,1991,47(8):819-826.
    [6]
    Milne PJ,Hunt AL,Rostoll K,et al.Medicinal chemistry:the biological activity of selected cyclic dipeptides[J].J Pharm Pharmacol,1998,50(12):1 331-1 337.
    [7]
    Li YX,Jiao WH,Zhou XC,et al.Secondary metabolites of Aspergillus aculeatus CGD12 isolated from mangrove and their antibacterial activities[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2013,19(19):71-76.
    [8]
    Fotakis G,Timbrell JA.In vitro cytotoxicity assays:comparison of LDH,neutral red,MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride[J].Toxicol Lett,2006,160(2):171-177.
    [9]
    Wynn TA,Ramalingam TR.Mechanisms of fibrosis:therapeutic translation for fibrotic disease[J].Nat Med,2012,18(7):1 028-1 040.
    [10]
    Fang F,Ooka K,Bhattachyya S,et al.The early growth response gene Egr2(Alias Krox20)is a novel transcriptional target of transforming growth factor-beta that is up-regulated in systemic sclerosis and mediates profibrotic responses[J].Am J Pathol,2011,178(5):2 077-2 290.
    [11]
    Barrientos S,Stojadinovic O,Golinko MS,et al.Growth factors and cytokines in wound healing[J].Wound Repair Regen,2008,16(5):585-601.
    [12]
    Gurtner GC,Werner S,Barrandon Y,et al.Wound repair and regeneration[J].Nature,2008, 453(7 193):314-321.
    [13]
    Fang F,Ooka K,Sun XY,et al.A synthetic TLR3 ligand mitigates profibrotic fibroblast responses by inducing autocrine IFN signaling[J].J Immunol,2013,191(6):2 956-2 966.
    [14]
    Werb Z.ECM and cell surface proteolysis:regulating cellular ecology[J].Cell,1997,91(4):439-442.
    [15]
    Wang H,Zhang Y,Heuckeroth RO.PAI-1 deficiency reduces liver fibrosis after bile duct ligation in mice through activation of tPA[J].Febs Letters,2007,581(16):3 098-3 104.
    • Related Articles

  • Related Articles

    [1]YE Zhenning, WU Zhenghong, ZHANG Huaqing. Research progress of blood-brain barrier crossing strategies and brain-targeted drug delivery mediated by nano-delivery system[J]. Journal of China Pharmaceutical University, 2024, 55(5): 590-602. DOI: 10.11665/j.issn.1000-5048.2024052202
    [2]YONG Qin, YUE Hanxun, SHI Min, HUANG Shiqin, ZHAO Xuan, YU Xian. Construction and in vitro evaluation of pH-responsive and tumor-targeted PTEN/PLGA-(HE)10-MAP nanoparticles[J]. Journal of China Pharmaceutical University, 2021, 52(3): 301-310. DOI: 10.11665/j.issn.1000-5048.20210306
    [3]FENG Yang, XU Xiao, MO Ran. Advances in lymphatic targeted drug delivery system for treatment of tumor metastasis[J]. Journal of China Pharmaceutical University, 2020, 51(4): 425-432. DOI: 10.11665/j.issn.1000-5048.20200406
    [4]SONG Ke, PAN Hao, HAN Jiayi, CHEN Lijiang. Nano drug delivery system based strategies to target tumor microenvironment[J]. Journal of China Pharmaceutical University, 2018, 49(4): 392-400. DOI: 10.11665/j.issn.1000-5048.20180402
    [5]FAN Qianqian, XING Lei, QIAO Jianbin, ZHANG Chenglu, JIANG Hulin. Advances in drug delivery systems for the treatment of liver fibrosis[J]. Journal of China Pharmaceutical University, 2018, 49(3): 263-271. DOI: 10.11665/j.issn.1000-5048.20180302
    [6]SUN Jingfang, GUO Chunjing, ZHANG Yunduan, SONG Xiaoyan, LYU Jiantao, YIN Jungang, CHEN Daquan. Preparation and characterization of multi-functional targeting nano-carrier based on pH-redox tumor microenvironment[J]. Journal of China Pharmaceutical University, 2017, 48(3): 305-310. DOI: 10.11665/j.issn.1000-5048.20170309
    [7]HE Wei, QI Haixia, DONG Lei, ZHANG Junfeng. Research advances in drug delivery system targeting immune system[J]. Journal of China Pharmaceutical University, 2015, 46(5): 513-520. DOI: 10.11665/j.issn.1000-5048.20150501
    [8]ZHANG Zhiqing, WANG Fang, ZHOU Ting, ZHANG Guodong, WANG Xiufeng, LI Yunze. A DNA polyaptamer system as a targeted antitumor drug delivery[J]. Journal of China Pharmaceutical University, 2015, 46(4). DOI: 10.11665/j.issn.1000-5048.20150408
    [9]SU Zhigui, MO Ran, ZHANG Can. Advances of nano-drug delivery systems overcoming the physiological and pathological barriers of tumor[J]. Journal of China Pharmaceutical University, 2015, 46(1): 28-39. DOI: 10.11665/j.issn.1000-5048.20150103
    [10]KE Xue. Advances in targeted drug delivery system[J]. Journal of China Pharmaceutical University, 2012, 43(1): 9-15.

Catalog

    Get Citation
    PDF
    XML
    Article views (882) PDF downloads (2573) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：327247

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return