Neuroprotective effect of glycyrrhizin on diabetic retinopathy

JIANG Xiaomei; LIU Chong

doi:10.11665/j.issn.1000-5048.20200610

Journal of China Pharmaceutical University > 2020 > 51(6): 711-717. > DOI: 10.11665/j.issn.1000-5048.20200610

JIANG Xiaomei, LIU Chong. Neuroprotective effect of glycyrrhizin on diabetic retinopathy[J]. Journal of China Pharmaceutical University, 2020, 51(6): 711-717. DOI: 10.11665/j.issn.1000-5048.20200610

Citation:

PDF (2747 KB)

Neuroprotective effect of glycyrrhizin on diabetic retinopathy

More Information

Received Date: January 19, 2020
Revised Date: July 08, 2020

Graphical Abstract

Abstract

Abstract

To explore the potential anti-inflammatory effects and retinal neuroprotective effects of glycyrrhizin (GL) on diabetic retinopathy (DR),twenty-one male C57BL/6 mice were randomly divided into control group,model group and glycyrrhizin (GL) group. The diabetic mice model was established by intraperitoneal injection of STZ. The GL group was treated with GL (150 mg/kg/d) by gavage for 6 weeks after modeling. Retinal tissue sections were paraffin-embedded and morphological examinated with hematoxylin-eosin (HE). Immunohistochemistry was used to detect the expression of GLUT1,GFAP and GAP43. RT-PCR was adopted to detect retinal inflammation and expression of apoptotic mediators (TNF-α,IL6,iNOS,NF-κB and Tp53). The results showed that the diabetic mice developed retinal disorders and retinal degeneration. The expression of glial cell proliferation marker GFAP was up-regulated,while the expression of neuronal plasticity marker GAP43 was down-regulated;NF-κB,TNF-α,IL6,iNOS and Tp53 transcription in the retina of diabetic mice increased. In the GL group,the degree of down-regulation of GLUT1 expression in the retina of diabetic mice was reduced,retinal inflammation was eliminated and the structure was improved. The above results suggested that GL may be a potential neuroprotective agent for diabetic patients and has anti-inflammatory effects,but its efficacy and safety in DR patients requires further clinical studies.
- glycyrrhizin,
- diabetics,
- retinopathy,
- anti-inflammatory

FullText(HTML)

References (18)

References

[1]	. Recent Advs Ophthalmol （眼科新进展），2018，38（12）：91?96.
[2]	Gubitosi-Klug RA. The diabetes control and complications trial/epidemiology of diabetes interventions and complications study at 30 years：summary and future directions［J］. Diabetes Care，2014，37（1）：44?49.
[3]	Morales-Sosa M，Orozco-Suarez S，Vega-Garcia A，et al. Immunomodulatory effect of Celecoxib on HMGB1/TLR4 pathway in a recurrent seizures model in immature rats［J］. Pharmacol Biochem Behav，2018，170（5）：79?86.
[4]	Wang L，Li YJ，Zhang b，et al. Protective effects and mechanism of glycyrrhizin on status epilepticus rats［J］. J Apoplexy Nerv Dis （中风与神经疾病杂志），2017，34（10）：897?901.
[5]	Liu L，Jiang Y，Steinle JJ. Inhibition of HMGB1 protects the retina from ischemia-reperfusion，as well as reduces insulin resistance proteins［J］. PLoS One，2017，12（5）：e0178236.
[6]	Nasiry D，Khalatbary AR，Ahmadvand H. Therapeutic potential of Juglans regia L. leaf extract against diabetic retinopathy in rat［J］. Iran J Basic Med Sci，2017，20（11）：1275?1281.
[7]	Kumagai AK，Glasgow BJ，Pardridge WM. GLUT1 glucose transporter expression in the diabetic and nondiabetic human eye［J］. Invest Ophth Vis Sci，1994，35（6）：2887?2894.
[8]	Kawasaki R，Konta T，Nishida K. Lipid-lowering medication is associated with decreased risk of diabetic retinopathy and the need for treatment in patients with type 2 diabetes：a real-world observational analysis of a health claims database［J］. Diabetes Obes Metab，2018，20（10）：2351?2360.
[9]	Elmasry K，Ibrahim AS，Saleh H，et al. Role of endoplasmic reticulum stress in 12/15-lipoxygenase-induced retinal microvascular dysfunction in a mouse model of diabetic retinopathy［J］. Diabetologia，2018，61（5）：1220?1232.
[10]	Bai Y，He Y，Yang LX. Mechanism of traditional Chinese medicine for promoting blood circulation and removing blood stasis in treating diabetic nephropathy［J］. Chin J Exp Tradit Med Form （中国实验方剂学杂志），2018，24（23）：200?206.
[11]	Dehdashtian E，Mehrzadi S，Yousefi B，et al. Diabetic retinopathy pathogenesis and the ameliorating effects of melatonin； involvement of autophagy，inflammation and oxidative stress［J］. Life Sci，2017，193（9）：20?33.
[12]	You ZP，Zhang YL，Shi K，et al. Suppression of diabetic retinopathy with GLUT1 siRNA［J］. Sci Rep，2017，7（1）：7437?7447.
[13]	Thorens B，Mueckler M. Glucose transporters in the 21st century［J］. Am J Physiol Endocrinol Metab，2010，298（2）：E141?E145.
[14]	Jianwei Z，Lingling W，Shucai L，et al. Expression changes of growth-associated protein-43 （GAP-43） and mitogen-activated protein kinase phosphatase-1 （MKP-1） and in hippocampus of streptozotocin-induced diabetic cognitive impairment rats［J］. Exp Neurol，2007，206（2）：201?208.
[15]	Li ZW，Liang HM，Li Z，et al. The protective effect of rosiglitazone on diabetic retinal Müller cells and its effects on the expression of glial fibrillary acidic protein （GFAP） and inflammatory factors in Müller cells［J］. Recent Advs Ophthalmol（眼科新进展），2018，38（9）：825?828.
[16]	Liu L， Jiang Y， Steinle JJ. Glycyrrhizin protects the diabetic retina against permeability，neuronal，and vascular damage through anti-Inflammatory mechanisms［J］. J Clin Med，2019，8（7）：957?963.
[17]	Chen JH. Neuroprotective effect and the mechanism of glycyrrhizin on hippocampal in infant rats with temporal lobe epilepsy（甘草甜素对幼鼠颞叶癫痫海马神经元的保护作用及其机制研究）［D］.Zhengzhou University（郑州大学），2017.
[18]	Holm TH，Dina D，Trevor O. Microglia are required for astroglial Toll-like receptor 4 response and for optimal TLR2 and TLR3 response［J］. Glia，2012，60（4）：630?638.

[1]	REN Weijie, CEN Lifang, ZOU Yi. Research progress of Bruton's tyrosine kinase (BTK) inhibitors in the treatment of inflammatory and immune-mediated diseases[J]. Journal of China Pharmaceutical University, 2024, 55(1): 63-72. DOI: 10.11665/j.issn.1000-5048.2023121103
[2]	WANG Chen, ZHANG Zhengping, LI Yinchun. Development strategy and clinical research progress of universal chimeric antigen receptor T-cell drugs[J]. Journal of China Pharmaceutical University, 2023, 54(2): 141-149. DOI: 10.11665/j.issn.1000-5048.20211125001
[3]	GAO Jing, MI Xue, ZHOU Qi, ZHOU Jun. Research progress of N⁶-methyladenine demethylase inhibitors[J]. Journal of China Pharmaceutical University, 2022, 53(6): 663-673. DOI: 10.11665/j.issn.1000-5048.20220604
[4]	ZHU Zhixi, ZHANG Jielin, CHEN Yijun. Recent advances in research on chelators as metallo-β-lactamase inhibitors[J]. Journal of China Pharmaceutical University, 2022, 53(4): 410-422. DOI: 10.11665/j.issn.1000-5048.20220404
[5]	YANG Qian, WANG Xiaojian. Research progress of sphingosine kinase 1 inhibitors[J]. Journal of China Pharmaceutical University, 2021, 52(6): 759-768. DOI: 10.11665/j.issn.1000-5048.20210615
[6]	LIU Xiaoqing, LIANG Shuang, LIU Yongjun, ZHANG Na. Progress of research on inhibition strategy of bromodomain-containing protein 4 and its application in tumor therapy[J]. Journal of China Pharmaceutical University, 2021, 52(3): 270-278. DOI: 10.11665/j.issn.1000-5048.20210302
[7]	WEI Yuanyuan, YANG Fan, TANG Jie, YU Lifang. Advances in the research of anti-tuberculosis drugs[J]. Journal of China Pharmaceutical University, 2020, 51(2): 231-239. DOI: 10.11665/j.issn.1000-5048.20200215
[8]	YUAN Zhong, CHEN Zhuo, LI Qianbin, HU Gaoyun. Advances in research of protein tyrosine phosphatase 1B and its inhibitors[J]. Journal of China Pharmaceutical University, 2018, 49(1): 1-9. DOI: 10.11665/j.issn.1000-5048.20180101
[9]	GUO Yahui, LU Peng, WANG Yubin, ZHANG Huibin. Progress in the researches for antitumor NEDD8 activating enzyme inhibitors[J]. Journal of China Pharmaceutical University, 2017, 48(6): 646-653. DOI: 10.11665/j.issn.1000-5048.20170603
[10]	YAO Aihong, CHANG Yujie, JIANG Cheng, SUN Haiying. Research progress of Polo-like kinase 1 inhibitors targeting Polo-box domain[J]. Journal of China Pharmaceutical University, 2016, 47(1): 1-8. DOI: 10.11665/j.issn.1000-5048.20160101

Cited By

Get Citation

PDF

XML

Article views (206) PDF downloads (640)

Turn off MathJax

Article Contents

Abstract

References

Neuroprotective effect of glycyrrhizin on diabetic retinopathy

Abstract

References

Related Articles

Catalog

Neuroprotective effect of glycyrrhizin on diabetic retinopathy

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content