Construction and <i>in vivo</i> evaluation of a thermosensitive hydrogel system loading with <i>Pseudomonas aeruginosa</i> DNA vaccine

SHI Min; YONG Qin; HE Yingna; HUANG Shiqin; ZHAO Xuan; YU Xian

doi:10.11665/j.issn.1000-5048.20210207

Journal of China Pharmaceutical University > 2021 > 52(2): 186-194. > DOI: 10.11665/j.issn.1000-5048.20210207

SHI Min, YONG Qin, HE Yingna, HUANG Shiqin, ZHAO Xuan, YU Xian. Construction and in vivo evaluation of a thermosensitive hydrogel system loading with Pseudomonas aeruginosa DNA vaccine[J]. Journal of China Pharmaceutical University, 2021, 52(2): 186-194. DOI: 10.11665/j.issn.1000-5048.20210207

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Construction and in vivo evaluation of a thermosensitive hydrogel system loading with Pseudomonas aeruginosa DNA vaccine

1.
Phase I Clinical Trial Center, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010
2.
College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200
3.
Department of Pharmacy, Central Hospital of Jiangjin District, Chongqing 402260, China

Funds: This study was supported by the National Natural Science Foundation of China (No. 82072327) and the Health Commission of Chongqing (No. 2016ZDXM010)

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Received Date: November 03, 2020
Revised Date: March 08, 2021

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Abstract

Abstract

A thermosensitive hydrogel system consisting of PLGA-PEG-PLGA hydrogel and Pseudomonas aeruginosa DNA vaccine was constructed and the immune efficacy of the system was evaluated. The PLGA-PEG-PLGA thermosensitive hydrogel containing Pseudomonas aeruginosa DNA vaccine was prepared by a simple physical mixing method. The gelation temperature, cytotoxicity, and release curve in vitro were tested.The degradability of hydrogel in vivo was evaluated. The mice were divided into control group (PBS), hydrogel group (Hydrogel), in vivo-jetPEI/plasmid DNA group (in vivo-jetPEI/pDNA), and hydrogel + in vivo-jetPEI/plasmid group (Gel+in vivo-jetPEI/pDNA). Mice were immunized three times with a ten-day interval. Two weeks after the last immunization, the mice were sacrificed. The proliferation of splenic lymphocytes, serum specific IgG antibodies and IFN-γ in supernatant of splenic lymphocytes were detected. The gelation temperature of PLGA-PEG-PLGA hydrogel was (32 ± 0.5) ℃. There was no obvious toxicity to cells in vitro, and about 80% of plasmid DNA was released after 7 days in vitro. PLGA-PEG-PLGA hydrogel was biodegradable, and degraded almost completely after 15 days in vivo. The spleen lymphocytes proliferated; the levels of specific IgG antibodies and IFN-γ of in vivo-jetPEI/pDNA and Gel+in vivo-jetPEI/pDNA groups increased. The hydrogel could enhance the immune response induced by DNA vaccine.Results suggest that the thermosensitive hydrogel system consisting of PLGA-PEG-PLGA hydrogel and Pseudomonas aeruginosa DNA vaccine is a promising new strategy for the development of PA vaccine.
- PLGA-PEG-PLGA,
- thermosensitive hydrogel,
- Pseudomonas aeruginosa,
- DNA vaccine,
- immune

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References

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